| Literature DB >> 23713894 |
Zhigang Yang1, Yanxia He, Jae-Hong Lee, Nayoung Park, Myungkoo Suh, Weon-Sik Chae, Jianfang Cao, Xiaojun Peng, Hyosung Jung, Chulhun Kang, Jong Seung Kim.
Abstract
A self-calibrating bipartite viscosity sensor 1 for cellular mitochondria, composed of coumarin and boron-dipyrromethene (BODIPY) with a rigid phenyl spacer and a mitochondria-targeting unit, was synthesized. The sensor showed a direct linear relationship between the fluorescence intensity ratio of BODIPY to coumarin or the fluorescence lifetime ratio and the media viscosity, which allowed us to determine the average mitochondrial viscosity in living HeLa cells as ca. 62 cP (cp). Upon treatment with an ionophore, monensin, or nystatin, the mitochondrial viscosity was observed to increase to ca. 110 cP.Entities:
Mesh：
Substances：
Year: 2013 PMID： 23713894 DOI： 10.1021/ja403851p
Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419