BACKGROUND: This study aimed to investigate the potential toxic effects of leached components from either a methacrylate-based composite (Filtek Z250) or a silorane-based composite (Filtek P90) on the male mice reproductive system. METHODS: Sixty adult Syrian male mice were divided into six groups. In test groups, leached components from composite specimens in artificial saliva or 75% aqueous ethanol solution were administered intragastrically daily for 28 days. The mice were then euthanized and the following reproductive parameters recorded: body weight changes; weight of paired testes; testis volume; Gonadosomatic Index (GSI); sperm motility; sperm viability; daily sperm production and epididymal sperm count. RESULTS: There were no significant differences in body weight changes, weight of paired testes, GSI, testis volume, epididymal sperm count, and daily sperm production between groups. Sperm motility and sperm viability were significantly lower in all the test groups in comparison to the control groups. In addition, they were significantly lower in the test groups in which composite samples were immersed in aqueous ethanol solution. CONCLUSIONS: Within the limitations of this study, the present data indicate that leached components from dental composites could affect sperm quality and therefore could potentially cause adverse effects on the male mice reproductive system.
BACKGROUND: This study aimed to investigate the potential toxic effects of leached components from either a methacrylate-based composite (Filtek Z250) or a silorane-based composite (Filtek P90) on the male mice reproductive system. METHODS: Sixty adult Syrian male mice were divided into six groups. In test groups, leached components from composite specimens in artificial saliva or 75% aqueous ethanol solution were administered intragastrically daily for 28 days. The mice were then euthanized and the following reproductive parameters recorded: body weight changes; weight of paired testes; testis volume; Gonadosomatic Index (GSI); sperm motility; sperm viability; daily sperm production and epididymal sperm count. RESULTS: There were no significant differences in body weight changes, weight of paired testes, GSI, testis volume, epididymal sperm count, and daily sperm production between groups. Sperm motility and sperm viability were significantly lower in all the test groups in comparison to the control groups. In addition, they were significantly lower in the test groups in which composite samples were immersed in aqueous ethanol solution. CONCLUSIONS: Within the limitations of this study, the present data indicate that leached components from dental composites could affect sperm quality and therefore could potentially cause adverse effects on the male mice reproductive system.