BACKGROUND AND AIMS: The large monophyletic genus Mimosa comprises approx. 500 species, most of which are native to the New World, with Central Brazil being the main centre of radiation. All Brazilian Mimosa spp. so far examined are nodulated by rhizobia in the betaproteobacterial genus Burkholderia. Approximately 10 Mya, transoceanic dispersal resulted in the Indian subcontinent hosting up to six endemic Mimosa spp. The nodulation ability and rhizobial symbionts of two of these, M. hamata and M. himalayana, both from north-west India, are here examined, and compared with those of M. pudica, an invasive species. METHODS: Nodules were collected from several locations, and examined by light and electron microscopy. Rhizobia isolated from them were characterized in terms of their abilities to nodulate the three Mimosa hosts. The molecular phylogenetic relationships of the rhizobia were determined by analysis of 16S rRNA, nifH and nodA gene sequences. KEY RESULTS: Both native Indian Mimosa spp. nodulated effectively in their respective rhizosphere soils. Based on 16S rRNA, nifH and nodA sequences, their symbionts were identified as belonging to the alphaproteobacterial genus Ensifer, and were closest to the 'Old World' Ensifer saheli, E. kostiensis and E. arboris. In contrast, the invasive M. pudica was predominantly nodulated by Betaproteobacteria in the genera Cupriavidus and Burkholderia. All rhizobial strains tested effectively nodulated their original hosts, but the symbionts of the native species could not nodulate M. pudica. CONCLUSIONS: The native Mimosa spp. in India are not nodulated by the Burkholderia symbionts of their South American relatives, but by a unique group of alpha-rhizobial microsymbionts that are closely related to the 'local' Old World Ensifer symbionts of other mimosoid legumes in north-west India. They appear not to share symbionts with the invasive M. pudica, symbionts of which are mostly beta-rhizobial.
BACKGROUND AND AIMS: The large monophyletic genus Mimosa comprises approx. 500 species, most of which are native to the New World, with Central Brazil being the main centre of radiation. All Brazilian Mimosa spp. so far examined are nodulated by rhizobia in the betaproteobacterial genus Burkholderia. Approximately 10 Mya, transoceanic dispersal resulted in the Indian subcontinent hosting up to six endemic Mimosa spp. The nodulation ability and rhizobial symbionts of two of these, M. hamata and M. himalayana, both from north-west India, are here examined, and compared with those of M. pudica, an invasive species. METHODS: Nodules were collected from several locations, and examined by light and electron microscopy. Rhizobia isolated from them were characterized in terms of their abilities to nodulate the three Mimosa hosts. The molecular phylogenetic relationships of the rhizobia were determined by analysis of 16S rRNA, nifH and nodA gene sequences. KEY RESULTS: Both native Indian Mimosa spp. nodulated effectively in their respective rhizosphere soils. Based on 16S rRNA, nifH and nodA sequences, their symbionts were identified as belonging to the alphaproteobacterial genus Ensifer, and were closest to the 'Old World' Ensifer saheli, E. kostiensis and E. arboris. In contrast, the invasive M. pudica was predominantly nodulated by Betaproteobacteria in the genera Cupriavidus and Burkholderia. All rhizobial strains tested effectively nodulated their original hosts, but the symbionts of the native species could not nodulate M. pudica. CONCLUSIONS: The native Mimosa spp. in India are not nodulated by the Burkholderia symbionts of their South American relatives, but by a unique group of alpha-rhizobial microsymbionts that are closely related to the 'local' Old World Ensifer symbionts of other mimosoid legumes in north-west India. They appear not to share symbionts with the invasive M. pudica, symbionts of which are mostly beta-rhizobial.
Authors: Wen-Ming Chen; Euan K James; Tom Coenye; Jui-Hsing Chou; Edmundo Barrios; Sergio M de Faria; Geoffrey N Elliott; Shih-Yi Sheu; Janet I Sprent; Peter Vandamme Journal: Int J Syst Evol Microbiol Date: 2006-08 Impact factor: 2.747
Authors: Wen-Ming Chen; Euan K James; Alan R Prescott; Martin Kierans; Janet I Sprent Journal: Mol Plant Microbe Interact Date: 2003-12 Impact factor: 4.171
Authors: Benny Lemaire; Samson B M Chimphango; Charles Stirton; Suhail Rafudeen; Olivier Honnay; Erik Smets; Wen-Ming Chen; Janet Sprent; Euan K James; A Muthama Muasya Journal: Appl Environ Microbiol Date: 2016-08-15 Impact factor: 4.792
Authors: Marco A Rogel; Patricia Bustos; Rosa I Santamaría; Víctor González; David Romero; Miguel Ángel Cevallos; Luis Lozano; Jaime Castro-Mondragón; Julio Martínez-Romero; Ernesto Ormeño-Orrillo; Esperanza Martínez-Romero Journal: BMC Genomics Date: 2014-07-08 Impact factor: 3.969
Authors: Lionel Moulin; Agnieszka Klonowska; Bournaud Caroline; Kristina Booth; Jan A C Vriezen; Rémy Melkonian; Euan K James; J Peter W Young; Gilles Bena; Loren Hauser; Miriam Land; Nikos Kyrpides; David Bruce; Patrick Chain; Alex Copeland; Sam Pitluck; Tanja Woyke; Michelle Lizotte-Waniewski; Jim Bristow; Margaret Riley Journal: Stand Genomic Sci Date: 2014-03-25