BACKGROUND: Banked unrelated donor umbilical cord blood (CB) has improved access to hematopoietic stem cell transplantation for patients without a suitably matched donor. In a resource-limited environment, ensuring that the public inventory is enriched with high-quality cord blood units (CBUs) addressing the needs of a diverse group of patients is a priority. Identification of donor characteristics correlating with higher CBU quality could guide operational strategies to increase the yield of banked high-quality CBUs. STUDY DESIGN AND METHODS: Characteristics of 5267 CBUs donated to the Carolinas Cord Blood Bank, a public bank participating in the National Cord Blood Inventory, were retrospectively analyzed. Eligible CBUs, collected by trained personnel, were processed using standard procedures. Routine quality and potency metrics (postprocessing total nucleated cell count [post-TNCC], CD34+, colony-forming units [CFUs]) were correlated with maternal, infant, and collection characteristics. RESULTS: High-quality CBUs were defined as those with higher post-TNCC (>1.25 × 10(9)) with CD34+ and CFUs in the upper quartile. Factors associated with higher CD34+ or CFU content included a shorter interval from collection to processing (<10 hr), younger gestational age (34-37 weeks; CD34+ and CFUs), Caucasian race, higher birthweight (>3500 g), and larger collection volumes (>80 mL). CONCLUSIONS: We describe characteristics identifying high-quality CBUs, which can be used to inform strategies for CBU collection for public banks. Efforts should be made to prioritize collections from larger babies born before 38 weeks of gestation. CBUs should be rapidly transported to the processing laboratory. The lower quality of CBUs from non-Caucasian donors highlights the challenges of building a racially diverse public CB inventory.
BACKGROUND: Banked unrelated donor umbilical cord blood (CB) has improved access to hematopoietic stem cell transplantation for patients without a suitably matched donor. In a resource-limited environment, ensuring that the public inventory is enriched with high-quality cord blood units (CBUs) addressing the needs of a diverse group of patients is a priority. Identification of donor characteristics correlating with higher CBU quality could guide operational strategies to increase the yield of banked high-quality CBUs. STUDY DESIGN AND METHODS: Characteristics of 5267 CBUs donated to the Carolinas Cord Blood Bank, a public bank participating in the National Cord Blood Inventory, were retrospectively analyzed. Eligible CBUs, collected by trained personnel, were processed using standard procedures. Routine quality and potency metrics (postprocessing total nucleated cell count [post-TNCC], CD34+, colony-forming units [CFUs]) were correlated with maternal, infant, and collection characteristics. RESULTS: High-quality CBUs were defined as those with higher post-TNCC (>1.25 × 10(9)) with CD34+ and CFUs in the upper quartile. Factors associated with higher CD34+ or CFU content included a shorter interval from collection to processing (<10 hr), younger gestational age (34-37 weeks; CD34+ and CFUs), Caucasian race, higher birthweight (>3500 g), and larger collection volumes (>80 mL). CONCLUSIONS: We describe characteristics identifying high-quality CBUs, which can be used to inform strategies for CBU collection for public banks. Efforts should be made to prioritize collections from larger babies born before 38 weeks of gestation. CBUs should be rapidly transported to the processing laboratory. The lower quality of CBUs from non-Caucasian donors highlights the challenges of building a racially diverse public CB inventory.
Authors: A Gasparoni; L Ciardelli; M A Avanzini; M Bonfichi; M di Mario; G Piazzi; L Martinotti; L Vanelli; G Rondini; G Chirico Journal: Pediatr Res Date: 2000-06 Impact factor: 3.756
Authors: J Kurtzberg; M Laughlin; M L Graham; C Smith; J F Olson; E C Halperin; G Ciocci; C Carrier; C E Stevens; P Rubinstein Journal: N Engl J Med Date: 1996-07-18 Impact factor: 91.245
Authors: A C M Luzo; A S S Duarte; T S I Salles; M L S Queiroz; I Lorand-Metze; F F Costa; S T O Saad Journal: Vox Sang Date: 2007-08 Impact factor: 2.144
Authors: Mitchell S Cairo; Elizabeth L Wagner; John Fraser; Geoff Cohen; Carmella van de Ven; Shelly L Carter; Nancy A Kernan; Joanne Kurtzberg Journal: Transfusion Date: 2005-06 Impact factor: 3.157
Authors: Mary Eapen; Pablo Rubinstein; Mei-Jie Zhang; Cladd Stevens; Joanne Kurtzberg; Andromachi Scaradavou; Fausto R Loberiza; Richard E Champlin; John P Klein; Mary M Horowitz; John E Wagner Journal: Lancet Date: 2007-06-09 Impact factor: 79.321
Authors: Thomas Bart; Michael Boo; Snejana Balabanova; Yvonne Fischer; Grazia Nicoloso; Lydia Foeken; Machteld Oudshoorn; Jakob Passweg; Andre Tichelli; Vincent Kindler; Joanne Kurtzberg; Thomas Price; Donna Regan; Elizabeth J Shpall; Rudolf Schwabe Journal: Transfus Med Hemother Date: 2013-01-07 Impact factor: 3.747
Authors: C Bordoni; J Magalon; C Gilbertas; M Gamerre; P Le Coz; M Berthomieu; C Chabannon; J Di Cristofaro; C Picard Journal: Bone Marrow Transplant Date: 2015-01-26 Impact factor: 5.483
Authors: Priya F Maillacheruvu; Lauren M Engel; Isaiah T Crum; Devendra K Agrawal; Eric S Peeples Journal: Pediatr Res Date: 2017-11-01 Impact factor: 3.756