Literature DB >> 23709030

Real-time monitoring the spatiotemporal dynamics of intracellular cGMP in vascular smooth muscle cells.

Kara F Held1, Wolfgang R Dostmann.   

Abstract

Real-time and noninvasive imaging of intracellular second messengers in mammalian cells, while -preserving their in vivo phenotype, requires biosensors of exquisite constitution. Here we provide the methodology for utilizing the single wavelength cGMP-biosensor δ-FlincG in aortic vascular smooth muscle cells.

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Year:  2013        PMID： 23709030      PMCID： PMC4887092          DOI： 10.1007/978-1-62703-459-3_8

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  56 in total

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Authors:  Viacheslav O Nikolaev; Stepan Gambaryan; Martin J Lohse
Journal:  Nat Methods       Date:  2006-01       Impact factor: 28.547

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Journal:  Endocrinology       Date:  1972-01       Impact factor: 4.736

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Journal:  Methods Enzymol       Date:  1996       Impact factor: 1.600

6.  Structural and functional features in human PDE5A1 regulatory domain that provide for allosteric cGMP binding, dimerization, and regulation.

Authors:  Roya Zoraghi; Emmanuel P Bessay; Jackie D Corbin; Sharron H Francis
Journal:  J Biol Chem       Date:  2005-01-27       Impact factor: 5.157

7.  Half-life of nitric oxide in aqueous solutions with and without haemoglobin.

Authors:  T S Hakim; K Sugimori; E M Camporesi; G Anderson
Journal:  Physiol Meas       Date:  1996-11       Impact factor: 2.833

8.  Regulator of G-protein signaling-2 mediates vascular smooth muscle relaxation and blood pressure.

Authors:  K Mary Tang; Guang-rong Wang; Ping Lu; Richard H Karas; Mark Aronovitz; Scott P Heximer; Kevin M Kaltenbronn; Kendall J Blumer; David P Siderovski; Yan Zhu; Michael E Mendelsohn; Mary Tang; Guang Wang
Journal:  Nat Med       Date:  2003-11-09       Impact factor: 53.440

9.  Rescue of cGMP kinase I knockout mice by smooth muscle specific expression of either isozyme.

Authors:  Silke Weber; Dominik Bernhard; Robert Lukowski; Pascal Weinmeister; René Wörner; Jörg W Wegener; Nadejda Valtcheva; Susanne Feil; Jens Schlossmann; Franz Hofmann; Robert Feil
Journal:  Circ Res       Date:  2007-09-27       Impact factor: 17.367

10.  Picomolar nitric oxide signals from central neurons recorded using ultrasensitive detector cells.

Authors:  Katherine C Wood; Andrew M Batchelor; Katalin Bartus; Kathryn L Harris; Giti Garthwaite; Jeffrey Vernon; John Garthwaite
Journal:  J Biol Chem       Date:  2011-10-20       Impact factor: 5.157
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