BACKGROUND: The aim of this study was to evaluate gene expression of bone remodeling markers in type 2 diabetic Goto-Kakizaki (GK) nonobese rats after gastrojejunal bypass and sleeve gastroplasty and their relationship with hormonal parameters. METHODS: We designed an experimental study in three groups of GK rats (nonoperated gastrojejunal bypass and sleeve gastroplasty). Gene expression of markers of bone remodeling and levels of insulin, leptin, and glucagon-like peptide-1 (GLP-1) were determined. RESULTS: GK rats had decreased levels of osteocalcin expression compared with Wistar rats. Gene expression of markers of bone remodeling in GK rats was similar in the three groups studied, although there was a trend to decreased receptor activator for nuclear factor κ B ligand (RANKL) in gastroplasty rats. Significant differences in the osteocalcin/RANKL ratio were observed between controls and gastrojejunal bypass rats compared with gastroplasty rats. The behavior of gastrointestinal hormones was antagonistic (GLP-1 gastrojejunal bypass 1.54 ± 0.24 ng/ml vs. GLP-1 gastroplasty 0.673 ± 0.09, p = 0.0001; leptin gastrojejunal bypass 1,178 ± 0.474 pg/ml vs. leptin gastroplasty 7,391 ± 4,054 pg/ml, p = 0.002). There was a reduction in leptin in the bypass group associated with an increase in gastrectomized rats. In gastrectomized rats, there was a trend toward an inverse relationship between leptin and RANKL (r = -0.771, p = 0.072). This relationship was more marked in the totality of operated rats, n = 12 (r = -0.608, p = 0.036). CONCLUSION: Our results show a more favorable profile of sleeve gastroplasty on bone remodeling. There was a trend to an increase in the expression of the osteocalcin gene, which is probably mediated by increased expression of leptin that inhibits the expression of RANKL.
BACKGROUND: The aim of this study was to evaluate gene expression of bone remodeling markers in type 2 diabetic Goto-Kakizaki (GK) nonobese rats after gastrojejunal bypass and sleeve gastroplasty and their relationship with hormonal parameters. METHODS: We designed an experimental study in three groups of GK rats (nonoperated gastrojejunal bypass and sleeve gastroplasty). Gene expression of markers of bone remodeling and levels of insulin, leptin, and glucagon-like peptide-1 (GLP-1) were determined. RESULTS: GK rats had decreased levels of osteocalcin expression compared with Wistar rats. Gene expression of markers of bone remodeling in GK rats was similar in the three groups studied, although there was a trend to decreased receptor activator for nuclear factor κ B ligand (RANKL) in gastroplasty rats. Significant differences in the osteocalcin/RANKL ratio were observed between controls and gastrojejunal bypass rats compared with gastroplasty rats. The behavior of gastrointestinal hormones was antagonistic (GLP-1 gastrojejunal bypass 1.54 ± 0.24 ng/ml vs. GLP-1 gastroplasty 0.673 ± 0.09, p = 0.0001; leptin gastrojejunal bypass 1,178 ± 0.474 pg/ml vs. leptin gastroplasty 7,391 ± 4,054 pg/ml, p = 0.002). There was a reduction in leptin in the bypass group associated with an increase in gastrectomized rats. In gastrectomized rats, there was a trend toward an inverse relationship between leptin and RANKL (r = -0.771, p = 0.072). This relationship was more marked in the totality of operated rats, n = 12 (r = -0.608, p = 0.036). CONCLUSION: Our results show a more favorable profile of sleeve gastroplasty on bone remodeling. There was a trend to an increase in the expression of the osteocalcin gene, which is probably mediated by increased expression of leptin that inhibits the expression of RANKL.
Authors: Raquel Cortes-Sancho; Jose L Perez-Castrillon; Juan C Martin-Escudero; Sonia Iglesias; Paula Alvarez-Manzanares; Rafael Ramos Journal: J Am Geriatr Soc Date: 2004-10 Impact factor: 5.562
Authors: João de Bona Castelan; Jane Bettiol; Armando José d'Acampora; João Vicente Edom Castelan; Jonathan Caon de Souza; Vinicius Bressiani; Simone Batisti Giroldi Journal: Obes Surg Date: 2007-07 Impact factor: 4.129
Authors: P Ducy; M Amling; S Takeda; M Priemel; A F Schilling; F T Beil; J Shen; C Vinson; J M Rueger; G Karsenty Journal: Cell Date: 2000-01-21 Impact factor: 41.582
Authors: Miriam A Bredella; Martin Torriani; Reza Hosseini Ghomi; Bijoy J Thomas; Danielle J Brick; Anu V Gerweck; Clifford J Rosen; Anne Klibanski; Karen K Miller Journal: Obesity (Silver Spring) Date: 2010-05-13 Impact factor: 5.002
Authors: Bernardo Nuche-Berenguer; Paola Moreno; Pedro Esbrit; Sonia Dapía; José R Caeiro; Jesús Cancelas; Juan J Haro-Mora; María L Villanueva-Peñacarrillo Journal: Calcif Tissue Int Date: 2009-02-15 Impact factor: 4.333
Authors: Russell T Turner; Satya P Kalra; Carmen P Wong; Kenneth A Philbrick; Laurence B Lindenmaier; Stephane Boghossian; Urszula T Iwaniec Journal: J Bone Miner Res Date: 2013-01 Impact factor: 6.741
Authors: José Luis Pérez-Castrillón; José Antonio Riancho; Daniel de Luis; José Ramón Caeiro; David Guede; Manuel González-Sagrado; Marta Ruiz-Mambrilla; María Domingo-Andrés; Rosa Conde; David Primo-Martín Journal: Obes Surg Date: 2012-11 Impact factor: 4.129
Authors: P Bergmann; J-J Body; S Boonen; Y Boutsen; J-P Devogelaer; S Goemaere; J-M Kaufman; J-Y Reginster; V Gangji Journal: Int J Clin Pract Date: 2009-01 Impact factor: 2.503
Authors: José A Balsa; Christian Lafuente; Jesús M Gómez-Martín; Julio Galindo; Roberto Peromingo; Francisca García-Moreno; Gloria Rodriguez-Velasco; Javier Martínez-Botas; Diego Gómez-Coronado; Héctor F Escobar-Morreale; José I Botella-Carretero Journal: J Bone Miner Metab Date: 2015-10-05 Impact factor: 2.626