| Literature DB >> 23707448 |
Zi-Guo Yuan1, Di Ren, Dong-Hui Zhou, Xiu-Xiang Zhang, Eskild Petersen, Xiu-Zhen Li, Yang Zhou, Gui-Lian Yang, Xing-Quan Zhu.
Abstract
Toxoplasma gondii is a significant zoonotic parasite which can cause congenital infection and abortion in warm-blooded animals and humans. Microneme protein 13 (MIC13) plays an important role in attachment and penetration of the host cell by T. gondii. In this study, a DNA vaccine expressing mic13 of T. gondii was constructed and its protective efficacy was evaluated in Kunming L615(H2k) mice. Immunization with pVAX-TgMIC13 induced a strong immune responses demonstrated by significant lymphocyte proliferation, cytokine production and antibody responses. Immunized mice showed increased survival time (21.3±11.3 days) and reduced number of cysts in brain of mice (57.14%) after challenge with tachyzoites of the virulent T. gondii RH strain and cysts of the T. gondii PRU strain, respectively, demonstrating that T. gondii MIC13 is a potential vaccine candidate, worth being included in future vaccine development against acute and chronic T. gondii infection.Entities:
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Year: 2013 PMID： 23707448 DOI： 10.1016/j.vaccine.2013.05.040
Source DB: PubMed Journal: Vaccine ISSN： 0264-410X Impact factor: 3.641