BACKGROUND: Attenuated Listeria monocytogenes vaccine strains have been administered intravenously (Le et al., Maciag et al.) and orally (Angelakopoulos et al., Johnson et al.) to humans. Here, one was given transcutaneously with cholera toxin adjuvant. METHODS:Eight healthy volunteers were studied (5 active, 3 placebo). Safety was assessed by physical exam and labs. Systemic immunological responses were measured by ELISA and IFN-gamma ELISpot. RESULTS: 4/5 active volunteers had cellular responses to listerial antigens. 5/5 active volunteers showed humoral responses to cholera toxin. CONCLUSIONS: An attenuated L. monocytogenes vector was safely administered transcutaneously. Topical administration appeared at least as immunogenic as previously studied oral delivery.
RCT Entities:
BACKGROUND: Attenuated Listeria monocytogenes vaccine strains have been administered intravenously (Le et al., Maciag et al.) and orally (Angelakopoulos et al., Johnson et al.) to humans. Here, one was given transcutaneously with cholera toxin adjuvant. METHODS: Eight healthy volunteers were studied (5 active, 3 placebo). Safety was assessed by physical exam and labs. Systemic immunological responses were measured by ELISA and IFN-gamma ELISpot. RESULTS: 4/5 active volunteers had cellular responses to listerial antigens. 5/5 active volunteers showed humoral responses to cholera toxin. CONCLUSIONS: An attenuated L. monocytogenes vector was safely administered transcutaneously. Topical administration appeared at least as immunogenic as previously studied oral delivery.
Authors: Dung T Le; Dirk G Brockstedt; Ran Nir-Paz; Johannes Hampl; Shruti Mathur; John Nemunaitis; Daniel H Sterman; Raffit Hassan; Eric Lutz; Bentley Moyer; Martin Giedlin; Jana-Lynn Louis; Elizabeth A Sugar; Alice Pons; Andrea L Cox; Jordana Levine; Aimee Luck Murphy; Peter Illei; Thomas W Dubensky; Joseph E Eiden; Elizabeth M Jaffee; Daniel A Laheru Journal: Clin Cancer Res Date: 2011-12-06 Impact factor: 12.531
Authors: Paul V Johnson; Barbra M Blair; Skye Zeller; Camille N Kotton; Elizabeth L Hohmann Journal: Microbiol Immunol Date: 2011-05 Impact factor: 1.955
Authors: Haroula Angelakopoulos; Katharina Loock; David M Sisul; Eric R Jensen; Jeff F Miller; Elizabeth L Hohmann Journal: Infect Immun Date: 2002-07 Impact factor: 3.441
Authors: Sarah A Frech; Richard T Kenney; Christian A Spyr; Hedvika Lazar; Jean-François Viret; Christian Herzog; Reinhard Glück; Gregory M Glenn Journal: Vaccine Date: 2005-01-04 Impact factor: 3.641
Authors: Xiaodong Jiang; Rachael A Clark; Luzheng Liu; Amy J Wagers; Robert C Fuhlbrigge; Thomas S Kupper Journal: Nature Date: 2012-02-29 Impact factor: 49.962
Authors: Fernando Güereña-Burgueño; Eric R Hall; David N Taylor; Frederick J Cassels; Daniel A Scott; Marcia K Wolf; Zachary J Roberts; Galina V Nesterova; Carl R Alving; Gregory M Glenn Journal: Infect Immun Date: 2002-04 Impact factor: 3.441