Using biomarkers in an evolutionary context: lessons from the analysis of biological responses of oligochaete annelids to metal exposure.

Anthropogenic activities may lead to the accumulation of inorganic and organic compounds in topsoils. Biota living in close contact with contaminated soils may experience stress at different levels of biological organization throughout the continuum from molecular to community level. Biological responses observed at the individual or infra-individual level of biological organization led to the development of biomarkers. The development of biomarkers consists often in evidencing biological modifications following a contaminant stress in laboratory conditions, using naïve organisms and it is sometime proposed to use the biological state of individuals from sentinel species collected in the field to evaluate the level of environmental exposure. However, considering the possibility of local adaptation following long-term exposure, organisms response sampled in the field may substantially differ from laboratory specimens. In this review, we discuss this point focusing on the definition and validity of molecular biomarkers of metal pollution using earthworms of the Lumbricidae family.