M Thenmozhi1, K Kannabiran, R Kumar, V Gopiesh Khanna. 1. Division of Biomolecules and Genetics, School of Biosciences & Technology, VIT University, Katpadi Road, Vellore 632 014, Tamil Nadu, India.
Abstract
OBJECTIVE: The aim of the present study was to assess the anti-Aspergillus activity of culture filtrate of Streptomyces sp. VITSTK7 and biologically synthesized Ag(2)O/Ag nanoparticles using the culture filtrate of VITSTK7. MATERIAL AND METHODS: Silver nanoparticles were synthesized by biological reduction of silver nitrate using culture filtrate of Streptomyces sp. VITSTK7. The synthesized nanopaticles were characterized for surface plasma resonance peak, X-ray diffraction patterns, shape and size distribution. Inhibition of mycelial growth was used as an index of anti-Aspergillus activity of synthesized nanoparticles. RESULTS: The synthesized nanoparticles were spherical shaped and monodespersive in nature and showed a single surface plasma resonance peak at 420 nm. X-ray diffraction patterns displayed typical peaks of crystalline silver oxide and size distribution histograms indicated production of 20-60-nm-size nanoparticles with average size of 35.2 nm. The synthesized nanoparticles showed anti-Aspergillus activity against Aspergillus niger, Aspergillus flavus and Aspergillus fumigatus with antifungal index in the range of 62-75%. CONCLUSION: Thus the bioconversion of Ag(2)O/Ag nanoparticles by Streptomyces sp. VITSTK7 could be employed to control Aspergillus pathogenesis. The results of this study suggest that the green synthesis of Ag(2)O/Ag nanoparticles using marine Streptomyces sp. VITSTK7 yielded 27.9 nm sized particles with potential to act against pathogenic Aspergillus species.
OBJECTIVE: The aim of the present study was to assess the anti-Aspergillus activity of culture filtrate of Streptomyces sp. VITSTK7 and biologically synthesized Ag(2)O/Ag nanoparticles using the culture filtrate of VITSTK7. MATERIAL AND METHODS:Silver nanoparticles were synthesized by biological reduction of silver nitrate using culture filtrate of Streptomyces sp. VITSTK7. The synthesized nanopaticles were characterized for surface plasma resonance peak, X-ray diffraction patterns, shape and size distribution. Inhibition of mycelial growth was used as an index of anti-Aspergillus activity of synthesized nanoparticles. RESULTS: The synthesized nanoparticles were spherical shaped and monodespersive in nature and showed a single surface plasma resonance peak at 420 nm. X-ray diffraction patterns displayed typical peaks of crystalline silver oxide and size distribution histograms indicated production of 20-60-nm-size nanoparticles with average size of 35.2 nm. The synthesized nanoparticles showed anti-Aspergillus activity against Aspergillus niger, Aspergillus flavus and Aspergillus fumigatus with antifungal index in the range of 62-75%. CONCLUSION: Thus the bioconversion of Ag(2)O/Ag nanoparticles by Streptomyces sp. VITSTK7 could be employed to control Aspergillus pathogenesis. The results of this study suggest that the green synthesis of Ag(2)O/Ag nanoparticles using marine Streptomyces sp. VITSTK7 yielded 27.9 nm sized particles with potential to act against pathogenic Aspergillus species.
Authors: Qaiser Shakeel; Ang Lyu; Jing Zhang; Mingde Wu; Guoqing Li; Tom Hsiang; Long Yang Journal: Front Microbiol Date: 2018-05-23 Impact factor: 5.640