OBJECTIVE: To determine the contents of lignans, crude polysaccharides (CP) and total phenolic compounds (TPC) of Schisandra chinensis from various habitats in Liaoning province and evaluate their quality and free radical scavenging (FRS) activity. METHODS: Contents of schisandrol, deoxyschizandrin and schisandrin B were determined by RP-HPLC. Contents of TPC, CP and FRS activity were determined by Folin-Cicalteu's, phenol-sulfuric acid and DPPH x method, respectively. RESULTS: Sample from Liaoyang city had the highest contents of lignans (21.75 mg/g); Sample from Shenbei New district of Shenyang city had the highest contents of CP (88.72 mg/g); Sample from Guanmenshan district of Benxi city had the highest contents of TPC and FRS activity (26.06 mg/g and 86.3%, respectively). Linear regression analysis results showed that contents of TPC had higher correlation coefficient with FRS activity than that of lignans. Their linear regression equations were Y = 1.3677X + 46.97, R2 = 0.6869 and Y = 2.5916X + 57.927, R2 = 0.1747 for TPC and lignans with FRS activity, respectively. CONCLUSION: The contents of lignans, CP and TPC are significantly different from samples collected from various habitats in Liaoning province. The main antioxidative substances are TPC, and lignans have no significant correlation with FRS activity.
OBJECTIVE: To determine the contents of lignans, crude polysaccharides (CP) and total phenolic compounds (TPC) of Schisandra chinensis from various habitats in Liaoning province and evaluate their quality and free radical scavenging (FRS) activity. METHODS: Contents of schisandrol, deoxyschizandrin and schisandrin B were determined by RP-HPLC. Contents of TPC, CP and FRS activity were determined by Folin-Cicalteu's, phenol-sulfuric acid and DPPH x method, respectively. RESULTS: Sample from Liaoyang city had the highest contents of lignans (21.75 mg/g); Sample from Shenbei New district of Shenyang city had the highest contents of CP (88.72 mg/g); Sample from Guanmenshan district of Benxi city had the highest contents of TPC and FRS activity (26.06 mg/g and 86.3%, respectively). Linear regression analysis results showed that contents of TPC had higher correlation coefficient with FRS activity than that of lignans. Their linear regression equations were Y = 1.3677X + 46.97, R2 = 0.6869 and Y = 2.5916X + 57.927, R2 = 0.1747 for TPC and lignans with FRS activity, respectively. CONCLUSION: The contents of lignans, CP and TPC are significantly different from samples collected from various habitats in Liaoning province. The main antioxidative substances are TPC, and lignans have no significant correlation with FRS activity.