Treatment effects on measures of body composition in the TODAY clinical trial.

OBJECTIVE: The Treatment Options for type 2 Diabetes in Adolescents and Youth (TODAY) trial showed superiority of metformin plus rosiglitazone (M+R) over metformin alone (M), with metformin plus lifestyle (M+L) intermediate in maintaining glycemic control. We report here treatment effects on measures of body composition and their relationships to demographic and metabolic variables including glycemia. RESEARCH DESIGN AND METHODS: Measures of adiposity (BMI, waist circumference, abdominal height, percent and absolute fat, and bone mineral content [BMC] and density [BMD]) were analyzed as change from baseline at 6 and 24 months.
RESULTS: Measures of fat accumulation were greatest in subjects treated with M+R and least in M+L. Although fat measures in M+L were less than those of M+R and M at 6 months, differences from M were no longer apparent at 24 months, whereas differences from M+R persisted at 24 months. The only body composition measure differing by race and/or ethnicity was waist circumference, greater in M+R than either M or M+L at both 6 and 24 months in whites. BMD and BMC increased in all groups, but increased less in M+R compared with the other two groups by 24 months. Measures of adiposity (increases in BMI, waist circumference, abdominal height, and fat) were associated with reduced insulin sensitivity and increased hemoglobin A1c (HbA1c), although effects of adiposity on HbA1c were less evident in those treated with M+R.
CONCLUSIONS: Despite differential effects on measures of adiposity (with M+R resulting in the most and M+L in the least fat accumulation), group differences generally were small and unrelated to treatment effects in sustaining glycemic control.

RCT Entities:   Population Interventions Outcomes

The recent increase in obesity among children and adolescents has been associated with an increased prevalence of type 2 diabetes in this population (1). The National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) sponsored a multicenter, randomized clinical trial to address treatment and progression of youth-onset type 2 diabetes. The primary objective of Treatment Options for type 2 Diabetes in Adolescents and Youth (TODAY) was to compare the ability of three treatments to maintain glycemic control. Treatment with metformin plus rosiglitazone (M+R) was superior to metformin alone (M) in preventing loss of glycemic control in youth with type 2 diabetes (2). Metformin plus an intensive lifestyle program (M+L) was not different from M or M+R. Neither BMI at baseline nor BMI over time was a determinant of treatment failure, although BMI (up to 60 months of treatment) increased most in M+R and least in M+L (P < 0.001). Analyses of the differential effects of treatment on fat and lean mass showed that subjects treated with M+R had the greatest increase in fat mass between baseline and 24 months. The current report presents a more in-depth analysis of BMI and other markers of adiposity by treatment group, including subgroup comparisons by sex and race/ethnicity. The analysis addresses whether changes in measures of body composition, including adiposity and bone, were associated with changes in measures of durable glycemic control in response to treatment.

RESEARCH DESIGN AND METHODS

TODAY sample and study design

The TODAY study rationale, design, and methods have been reported in detail (3) and are described briefly. Between July 2004 and February 2009, 699 youth were enrolled that met the following criteria: 10–17 years of age, diagnosed with type 2 diabetes <2 years according to American Diabetes Association (ADA) diagnostic glucose criteria (4), BMI ≥85th percentile, and negative for diabetes autoantibodies (5). Subjects also had to successfully complete a 2–6 month prerandomization run-in period in which they demonstrated mastery of standard diabetes education, were weaned from nonstudy diabetes medications, demonstrated tolerance of metformin up to a dose of 1,000 mg twice daily but no less than 500 mg twice daily, attained glycemic control (HbA1c <8% [64 mmol/mol] monthly for at least 2 months) on metformin alone, and demonstrated adherence to study medication and visit attendance (6). The primary objective was to compare the three treatment arms (M, M+R, and M+L) on time to treatment failure, i.e., loss of glycemic control defined as either HbA1c ≥8% (64 mmol/mol) over a 6-month period or inability to wean from temporary insulin therapy within 3 months after acute metabolic decompensation. After treatment failure, rosiglitazone was stopped, if applicable, and participants continued on metformin plus insulin for diabetes management. The protocol was approved by an external evaluation committee convened by the NIDDK and the institutional review board of each participating institution. All participants provided both informed parental consent and minor child assent.

Anthropometric outcomes

Staff were trained and certified to perform all measures. Height and weight were collected at all visits and waist circumference and abdominal height annually. Height measurements were made using a clinical stadiometer, and weight was measured in duplicate using a Seca scale (model 882; seca, Hanover, MA) with a third measurement obtained if the first measurements differed >0.2 kg. Scales and stadiometers were calibrated at least every 8 weeks, and scale accuracy was checked against 150-lb weights every 4 weeks. BMI was calculated as weight (kg)/height2 (m2). Waist circumference (cm) was measured at the iliac crest during maximum exhalation using a nonstretch, nontension fiberglass Gulick II tape measure positioned horizontally to the floor according to the U.S. Department of Health and Human Services, Public Health Service, National Health and Nutrition Examination Survey III Anthropometric Procedures (http://www.ncbi.nlm.nih.gov/books/NBK2004/). Abdominal height (cm) was measured laterally with the patient supine using a Holtain Kahn abdominal caliper (8). Measurements were taken midabdominally between the right and left iliac crest.

Dual X-ray absorptiometry outcomes

Dual X-ray absorptiometry (DXA) scans were performed using the existing densitometry system at each clinical center (models QDR4500A, Discovery A, Discovery W/Wi, Delphi W, and Delphi A from Hologic Inc., Bedford, MA; models Prodigy and iDXA from GE Lunar Corp, Madison, WI; model DPX-IQ from Lunar Corp, Madison, WI). All scans were performed according to study-specific guidelines for subject positioning standardized across the different DXA systems. DXA quality assurance procedures included phantom cross-calibration and longitudinal monitoring (9,10). Although there are well-known systematic differences in the absolute values of these systems, the change in the Hologic and GE-Lunar body composition values (i.e., relative results to baseline) is comparable because of the demonstrated linear relationship across a wide range of ages for both sexes (11). All scans were analyzed centrally at the TODAY DXA Central Reading Center (University of California at San Francisco) by study-trained personnel using software according to manufacturer guidelines. The software versions for analyzing the scans were Hologic Discovery 12.3 for the Hologic scans, Prodigy 11.4 for the Prodigy and iDXA scans, and GE-Lunar 4.7e for the DPX-L scans. About one-quarter of the DXA scans could not be used in the analysis; scans were invalidated due to either weight and size limitations set by the equipment manufacturers or because a body part (e.g., arm or leg) was completely or partially off the scanner, there was hand-hip overlap, or there was movement during the scan (specific reasons for missing scan data were not recorded during the trial).

Statistical methods

Analyses were performed on data collected at baseline (n = 699), month 6 (n = 596), and month 24 (n = 403), which were the major outcome data collection visits including DXA. Only data collected prior to the occurrence of the primary outcome (failure to maintain glycemic control) were included since management with insulin, which was started after treatment failure, is known to change measures of body composition.

A mixed effects repeated measures analysis that appropriately accounted for the longitudinal data structure was used to analyze change from baseline. The method used all data available for each participant at time points prior to treatment failure. The model included treatment, visit, and treatment-by-visit interaction, with the value of the outcome at baseline as a covariate. Models of bone mineral content (BMC) and bone mineral density (BMD) also adjusted for height and age at the time of visit. If the treatment-by-visit interaction was significant, we examined pairwise treatment differences at 6 and 24 months. Model-adjusted means (least squares means [LSmeans]) and SEs of the change from baseline are reported in the tables and the graphs. Subgroup differences across treatments were modeled, including an interaction term for treatment by sex or treatment by race/ethnicity. Results are given for the three categories of race/ethnicity with acceptable sample size (non-Hispanic black [NHB], Hispanic [H], and non-Hispanic white [NHW]). The relationships between change from baseline insulin sensitivity (1/fasting insulin in μU/mL) or HbA1c and measures of body composition included the baseline value of either insulin sensitivity or HbA1c as a covariate.

All analyses were considered exploratory, with statistical significance defined as P < 0.05 and no adjustment for multiple testing; the study was powered for the primary study outcome only. The Statistical Analysis Software package (SAS, version 9.2, 2008; SAS Institute Inc., Cary, NC) was used for all analyses.

RESULTS

The TODAY cohort at baseline (7) is described as follows: average age 14.0 (SD 2.0) years, duration of type 2 diabetes 7.8 (SD 5.9) months, mostly pubertal (88.7% Tanner stage 4 or 5), 64.7% female, 32.5% NHB, 39.7% H, 20.3% NHW, 5.9% American Indian, and 1.6% Asian. Almost half of the cohort (n = 319, 45.6%) reached the primary outcome after an average follow-up duration of 3.9 years (range, 2–6.5 years); the M+R combination was superior to M (P = 0.006), and M+L was intermediate but not different from M or M+R (2).

Supplementary Table 1 includes raw means and SDs for the five adiposity measures (BMI, waist circumference, abdominal height, percent, and absolute fat mass) and the two DXA bone measures (BMC and BMD) by study visit (baseline and 6 and 24 months) and treatment group; part A includes output for the overall sample and by sex and part B by racial/ethnic subgroup for NHB, H, and NHW.

The outcomes were moderately to highly intercorrelated: r > 0.8 among BMI, waist circumference, absolute fat mass, and abdominal height; r ≈ 0.5 for percent fat mass with BMI, waist circumference, and abdominal height; r ≈ 0.7 for percent fat mass with absolute fat mass; and r > 0.8 between BMC and BMD. In addition, despite statistical significance, the absolute magnitude was extremely small for most of the body composition measures reported.

Treatment effects on indices of adiposity

Table 1 includes change from baseline data by treatment group assignment; Fig. 1 shows the trends for BMI and percent fat graphically. All changes in measures of adiposity with treatment, and all differences between treatment groups, even if statistically significant, were small. During the first 6 months of treatment, all measures of adiposity declined in the M+L group but increased slightly in the other groups. Change in the M+L group was significantly lower than in M+R for four of the five measures (BMI, percent fat mass, abdominal height, and absolute fat mass) and lower than in M for three (BMI, percent fat mass, and absolute fat mass); M was not significantly different from M+R. By 24 months, all measures of adiposity had increased relative to baseline in all treatment groups except for percent fat mass in M+L; all changes were significantly greater in M+R than in either M or M+L, and there were no statistically significant differences between M and M+L.

Table 1

Body composition model-adjusted mean* (SE) change from baseline and significant treatment group comparisons

Figure 1

LSmean change from baseline for BMI (A) and percent fat mass (B) at 6 and 24 months, by treatment. Treatment groups are denoted by M for metformin alone, M+R for metformin + rosiglitazone, and M+L for metformin + intensive lifestyle intervention. LSmeans and P values are output from an analysis modeling BMI and percent fat mass change from baseline (6 − 0 months and 24−0 months) as a function of baseline value, treatment, period (0–6 or 0–24 months), and the interaction of treatment by period. Significant treatment group differences for the 6- and 24-month change from baseline are indicated within the figure.

Treatment effects by sex and race/ethnicity

There was no treatment-by-sex interaction for any adiposity indicator. The only measure of adiposity that showed a significant treatment effect by race/ethnicity was waist circumference (P = 0.0205) (Supplementary Fig. 1). In NHW, the increase in waist circumference for M+R was significantly greater compared with that of M and M+L at both 6 (P = 0.0006 and P = 0.0041) and 24 months (P < 0.0001 and P = 0.0006; M and M+L, respectively). Within subjects treated with M+R, the increase in waist circumference was significantly greater in NHW compared with H at both 6 (P = 0.0404) and 24 (P = 0.0454) months; within subjects treated with M, significant differences between NHW and H (P = 0.0095) and NHW and NHB (P = 0.0106) at 6 months had disappeared by 24 months.

Treatment effects on bone

Both BMD and BMC increased from baseline in all groups (Table 1 and Fig. 2), but by 24 months, the increase in BMD in the M+R group was significantly less than that of the M+L group (P = 0.0041). The trend was similar in BMC, although it did not reach statistical significance (P = 0.0670).

Figure 2

LSmean change from baseline for BMC (g) (A) and BMD (g/cm2) (B) at 6 and 24 months, by treatment. Treatment groups are denoted by M, M+R, and M+L. LSmeans and P values are output from an analysis modeling BMC and BMD change from baseline (6–0 and 24–0) as a function of baseline value, treatment, period (0–6 or 0–24 months), height, and age at the time of visit, and the interaction of treatment by period. Significant treatment group differences for the 6- and 24-month change from baseline are indicated within the figure (nonsignificant differences are indicated by NS).

Associations with insulin sensitivity and HbA1c

BMI change from baseline (Fig. 3) was associated with significant and consistent changes in a positive direction for HbA1c (as BMI increases so does HbA1c) and in a negative direction for insulin sensitivity (as BMI increases, insulin sensitivity decreases). In general, in the M+R group, fat accumulation had less of an effect on increasing HbA1c compared with the other treatment groups, as treatment with M+R appeared to ameliorate the effect of increased adiposity on HbA1c. As shown in Fig. 3, M+R blunted the effect of adiposity (as indicated by BMI) on HbA1c relative to that seen with M and M+L. This effect was significant at 6 months (P = 0.0109, M+R vs. M+L; P = 0.0253, M+R vs. M) but not at 24 months.

Figure 3

Regression lines of change from baseline for insulin sensitivity (1/IF µU/mL) versus BMI change, from baseline to 6 months (A) and baseline to 24 months (B); and regression lines of change from baseline for HbA1c (%) vs. BMI change, from baseline to 6 months (C) and baseline to 24 months (D), by treatment. Treatment groups are denoted by M, M+R, and M+L. The horizontal axis scales represent the 5th–95th percentiles of the distribution of BMI change from baseline (−3 to 3 at 6 months and −4 to +7 at 24 months). Note that the 5th–95th percentile range of BMI change from baseline is greater for the 24-month period than for the 6-month one. Slopes indicate change in insulin sensitivity or HbA1c per unit increase in BMI change and were evaluated from models including the baseline value of either insulin sensitivity or HbA1c as a covariate.

CONCLUSIONS

During the design of the TODAY trial, we hypothesized that treatment with M+L would result in the greatest reductions in adiposity, that treatment with M+R would result in the greatest weight gain, and that increased adiposity would be associated with a deterioration in glycemic control. Data presented herein indicate that the treatment group differences reported (2) in the primary outcome analysis (superiority of M+R in sustaining glycemic control compared with M, with M+L intermediate) are not explained by concordant differences in BMI or whole-body adiposity. These data also indicate that treatment with M+R was associated not merely with weight gain but also with more fat deposition than M or M+L treatment. Although treatment with M+L was associated with reductions in most measures of adiposity at 6 months after the start of treatment, these effects generally were modest and no longer detected at 24 months.

It was not surprising that increases in measures of adiposity were observed in the M+R group during the course of treatment, since similar effects of thiazolidinediones (TZDs, the drug class that encompasses rosiglitazone) are well described in adults (12–16). These observations in adults suggest that the beneficial effects of TZDs on glycemia are not mediated through an improvement in insulin sensitivity expected from reduced adiposity. Indeed despite its superiority in maintaining durable glycemic control, treatment with M+R in this study resulted in substantially more adiposity, as indicated by a variety of measures (with an absolute mean increase in BMI of 2.93 kg/m2 in the M+R group over 24 months), compared with treatment with the other modalities. It should be noted, however, that increased weight and fat gain with TZD treatment have not been universally found in children (17–19).

Nor was it surprising that improved indices of body composition in the M+L group were most evident during the first 6 months of treatment, a time of frequent and close contact between the participant and the TODAY lifestyle intervention team. These data are similar to those from other adult and pediatric cohorts in which lifestyle treatment has been shown to be most effective during the initial, high-contact phase (20,21).

It is less clear why the significant improvements in most measures of body composition during M+L did not translate into sustained glycemic control. As noted, the changes in all measures of adiposity were small, and the favorable changes in the M+L group at 6 months were no longer significantly different at 24 months. It is possible that the favorable changes in body composition observed in the M+L group simply were not of sufficient magnitude or duration to be translated into maintenance of glycemic control. Although the amount of weight loss required to effect glycemic control in growing adolescents is not known, the observed losses in measures of adiposity in the M+L treatment group were below thresholds reported to have an effect on HbA1c in the adult literature (22).

BMD and BMC accretion occurred in all treatment groups over time as expected in this age-group (23). Worth emphasizing, however, is the fact that the change in BMD from baseline to 24 months was less in the M+R group than in the M+L group, with a similar but nonsignificant trend seen in BMC. Since TZD treatment has been associated with increased rate of bone loss and fractures (24), it is possible that these relative reductions in BMD, albeit small, may translate over time to reduced bone strength. It is unknown whether TZD effects on bone are confined to the period of treatment, or whether effects continue after TZD exposure is stopped.

Indices of adiposity generally were associated with inverse changes in insulin sensitivity, confirming that adiposity is a key modulator of insulin sensitivity. Indices of adiposity also were associated with concordant changes in HbA1c, effects that could be partially overcome by M+R treatment. Whether these relationships between treatment and adiposity are common to all youth with type 2 diabetes or reflect in some way the extreme adiposity of our cohort (mean 97.7 BMI percentile at baseline) remains unclear.

Several limitations in our data are worth noting. First, all indices of adiposity measured were moderately to strongly correlated; thus it was not surprising that most treatment group effects were consistent for all measures. However, the various measures selected do provide slightly different reflections of adiposity, and this analysis gives no clear indication of selective regional effects. Second, it is well known that visceral adiposity is more predictive of cardiovascular risk and insulin resistance (25,26) than total body fat, and visceral fat was not measured directly in this study. However two surrogate markers (waist circumference and abdominal height) (27–29) were measured and both increased the most in the M+R group. In this regard, our data closely resemble those from A Diabetes Outcome Progression Trial (ADOPT) study in which rosiglitazone treatment was associated with better glycemic control than metformin alone, despite increases in total body weight, body fat, and waist circumference (30). Although speculative, a redistribution of abdominal fat by TZD treatment from visceral to subcutaneous fat depots, as suggested in previous studies (14,31), cannot be discounted. Third, ∼25% of DXA scans were invalid or could not be performed for a variety of reasons, including weight limitations, thus possibly introducing some degree of sampling bias. It is likely, however, that such bias would only have reduced our ability to discriminate between treatment group differences in body composition, especially since the M+R participants were heavier, and thus more likely to have been excluded from DXA measurements because of weight restrictions. Finally, it is important to consider the magnitude of the differences noted in this manuscript. Although statistically significant, the absolute magnitude of all changes reported is very small, which raises the question of biological relevance despite statistical significance in these measures of body composition.

In summary, these findings indicate that the variable times to glycemic failure by treatment group observed in the TODAY trial were not explained by concordant treatment effects on measures of body composition. In the primary outcome analysis, the treatment failure rate for NHB (52.9%) was greater than that for NHW (36.9%) or H (44.8%), with the highest failure rates observed in NHB participants treated with M alone (2). From the current analysis, it is evident that race/ethnicity differences in changes in adiposity measures do not account for these race/ethnicity differences in treatment responses, since the rates of fat accumulation in NHB participants, including those treated with M, were no greater than those of the other race/ethnic groups. It can be concluded that additional factors other than reductions in BMI or adiposity are responsible for some of the differences observed between treatment groups in the TODAY study in maintaining glycemic control. We have shown previously that adherence to treatment did not account for the primary outcome results (2). Future studies, including the long-term follow-up of the TODAY cohort, may help clarify whether the differential effects of diabetes treatment seen in this study are most related to the basic underlying mechanisms of the drugs used, or to genetic, social, biological, or lifestyle factors.