Exploring the differences between mouse mAβ(1-42) and human hAβ(1-42) for Alzheimer's disease related properties and neuronal cytotoxicity.

The differences between mouse mAβ(1-42) and human hAβ(1-42), explored using CD and fluorescence spectroscopy, transmission electron microscopy, ROS fluorescent assay, and neuronal cell viability, revealed that mAβ(1-42) as a three-site mutant (R5G, Y10F and H13R) of hAβ(1-42) altered the metal (copper and zinc) binding sites, reduced the proneness to form β-sheet structures and aggregated fibrils, alleviated the generation of ROS, and decreased the cytotoxicity, in contrast to hAβ(1-42).