PURPOSE: To investigate transient receptor potential vanilloid 4 (TRPV4) expression and the effects of ruthenium red (RR)-TRPV antagonist-on detrusor overactivity (DO) associated with bladder outlet obstruction (BOO). METHODS: Rats were randomly assigned to 3 groups. The control group (n = 10) included sham-operated rats. The BOO-group without RR (n = 15) and BOO-group with RR (n = 15) underwent partial BOO surgery. Three weeks postoperatively, cystometrography was performed in all rats. After confirming DO, RR was instilled intravesically in the BOO-group with RR. Urodynamic parameters were investigated, including contraction interval (CI) and contraction pressure (CP). TRPV4 expression was evaluated through immunofluorescence staining and western blotting. RESULTS: The BOO-group without RR had significantly shorter CI and significantly higher CP compared to the control. In the BOO-group with RR, CI was significantly longer compared to the BOO-group without RR. However, change in CP between BOO-group without and with RR was not significantly different. Immunofluorescence staining showed that TRPV4 was localized in the urothelium and detrusor muscles. TRPV4 immunofluorescence signals were increased in the urothelium and detrusor muscle in BOO-group without RR, compared with the control. In western blot analysis, immunoreactive bands indicating expression of TRPV4 were detected in the urothelium and detrusor muscle, and those were significantly increased in the BOO-group without RR compared with the control in the urothelium and detrusor muscle. CONCLUSIONS: TRPV4 plays an important role in the pathophysiology of DO, and RR has a beneficial effect on DO associated with BOO.
PURPOSE: To investigate transient receptor potential vanilloid 4 (TRPV4) expression and the effects of ruthenium red (RR)-TRPV antagonist-on detrusor overactivity (DO) associated with bladder outlet obstruction (BOO). METHODS:Rats were randomly assigned to 3 groups. The control group (n = 10) included sham-operated rats. The BOO-group without RR (n = 15) and BOO-group with RR (n = 15) underwent partial BOO surgery. Three weeks postoperatively, cystometrography was performed in all rats. After confirming DO, RR was instilled intravesically in the BOO-group with RR. Urodynamic parameters were investigated, including contraction interval (CI) and contraction pressure (CP). TRPV4 expression was evaluated through immunofluorescence staining and western blotting. RESULTS: The BOO-group without RR had significantly shorter CI and significantly higher CP compared to the control. In the BOO-group with RR, CI was significantly longer compared to the BOO-group without RR. However, change in CP between BOO-group without and with RR was not significantly different. Immunofluorescence staining showed that TRPV4 was localized in the urothelium and detrusor muscles. TRPV4 immunofluorescence signals were increased in the urothelium and detrusor muscle in BOO-group without RR, compared with the control. In western blot analysis, immunoreactive bands indicating expression of TRPV4 were detected in the urothelium and detrusor muscle, and those were significantly increased in the BOO-group without RR compared with the control in the urothelium and detrusor muscle. CONCLUSIONS:TRPV4 plays an important role in the pathophysiology of DO, and RR has a beneficial effect on DO associated with BOO.
Authors: M Vlaskovska; L Kasakov; W Rong; P Bodin; M Bardini; D A Cockayne; A P Ford; G Burnstock Journal: J Neurosci Date: 2001-08-01 Impact factor: 6.167
Authors: Dick A W Janssen; Joost G Hoenderop; Kees C F J Jansen; Annemiete W Kemp; John P F A Heesakkers; Jack A Schalken Journal: J Urol Date: 2011-07-23 Impact factor: 7.450
Authors: Lori Birder; F Aura Kullmann; Hyosang Lee; Stacey Barrick; William de Groat; Anthony Kanai; Michael Caterina Journal: J Pharmacol Exp Ther Date: 2007-07-17 Impact factor: 4.030
Authors: Francis M Hughes; Hayden M Hill; Case M Wood; Andrew T Edmondson; Aliya Dumas; Wen-Chi Foo; James M Oelsen; Goran Rac; J Todd Purves Journal: J Urol Date: 2015-12-18 Impact factor: 7.450
Authors: Andreas Hammer; Geraldine Cerretti; Dario A Ricciardi; David Schiffmann; Simon Maranda; Raphael Kummer; Christoph Zumbühl; Karin F Rattenbacher-Kiser; Silvan von Arx; Sebastian Ammann; Frederic Strobl; Rayene Berkane; Alexandra Stolz; Ernst H K Stelzer; Marcel Egli; Enrico Schleiff; Simon L Wuest; Maik Böhmer Journal: Biomedicines Date: 2022-01-08