Literature DB >> 23698523

Simplified serum neutralization test based on enhanced green fluorescent protein-tagged classical swine fever virus.

Yongfeng Li1, Liang Shen, Yuan Sun, Jin Yuan, Junhua Huang, Chao Li, Su Li, Yuzi Luo, Hua-Ji Qiu.   

Abstract

The neutralization immunofluorescence test (NIFT), currently used for detecting neutralizing antibodies (NAbs) against classical swine fever virus (CSFV), is time-consuming. Here, a simplified neutralization test based on enhanced green fluorescent protein (EGFP)-tagged CSFV (EGFP-NT) was developed for direct detection of anti-CSFV NAbs without immunostaining. The relative sensitivity and specificity between EGFP-NT and NIFT or blocking enzyme-linked immunosorbent assay (ELISA) were both 100%. The NAb titers by EGFP-NT and the blocking rates by blocking ELISA showed a good correlation.

Entities:  

Mesh：

Substances：

Year:  2013        PMID： 23698523      PMCID： PMC3719638          DOI： 10.1128/JCM.00859-13

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  16 in total

1.  A direct neutralizing peroxidase-linked antibody assay for detection and titration of antibodies to bovine viral diarrhoea virus.

Authors:  J M Hyera; B Liess; H R Frey
Journal:  Zentralbl Veterinarmed B       Date:  1987-04

2.  An improved ELISA for the detection of serum antibodies directed against classical swine fever virus.

Authors:  E O Colijn; M Bloemraad; G Wensvoort
Journal:  Vet Microbiol       Date:  1997-12       Impact factor: 3.293

3.  An enzyme immunoassay employing monoclonal antibodies and detecting specifically antibodies to classical swine fever virus.

Authors:  G Wensvoort; M Bloemraad; C Terpstra
Journal:  Vet Microbiol       Date:  1988-06       Impact factor: 3.293

4.  [Differential diagnosis of classical swine fever and border disease: seroepidemiological investigation of a pestivirus infection on a mixed sheep and swine farm].

Authors:  T C Oguzoglu; G Floegel-Niesmann; H R Frey; V Moennig
Journal:  Dtsch Tierarztl Wochenschr       Date:  2001-05

5.  A rapid serum neutralization test in microplates for the detection of antibodies to hog cholera virus.

Authors:  C Buonavoglia; E Falcone; S Pestalozza; L Di Trani; E D'Amore
Journal:  J Virol Methods       Date:  1989-01       Impact factor: 2.014

6.  A new assay for classical swine fever virus based on cytopathogenicity in porcine kidney cell line FS-L3.

Authors:  Y Sakoda; O Yamaguchi; A Fukusho
Journal:  J Virol Methods       Date:  1998-01       Impact factor: 2.014

7.  The neutralizing peroxidase-linked assay for detection of antibody against swine fever virus.

Authors:  C Terpstra; M Bloemraad; A L Gielkens
Journal:  Vet Microbiol       Date:  1984-04       Impact factor: 3.293

8.  The protective value of vaccine-induced neutralising antibody titres in swine fever.

Authors:  C Terpstra; G Wensvoort
Journal:  Vet Microbiol       Date:  1988-02       Impact factor: 3.293

9.  Expression and stability of foreign tags inserted into nsp2 of porcine reproductive and respiratory syndrome virus (PRRSV).

Authors:  Dal-Young Kim; Jay G Calvert; Kyeong-Ok Chang; Kyle Horlen; Maureen Kerrigan; Raymond R R Rowland
Journal:  Virus Res       Date:  2007-06-05       Impact factor: 3.303

10.  Efficient and stable rescue of classical swine fever virus from cloned cDNA using an RNA polymerase II system.

Authors:  Chao Li; Junhua Huang; Yongfeng Li; Fan He; Dan Li; Yuan Sun; Wen Han; Su Li; Hua-Ji Qiu
Journal:  Arch Virol       Date:  2012-11-29       Impact factor: 2.574
View more
  4 in total

1.  A recombinant avian leukosis virus subgroup j for directly monitoring viral infection and the selection of neutralizing antibodies.

Authors:  Qi Wang; Xiaofei Li; Xiaolin Ji; Jingfei Wang; Nan Shen; Yulong Gao; Xiaole Qi; Yongqiang Wang; Honglei Gao; Shide Zhang; Xiaomei Wang
Journal:  PLoS One       Date:  2014-12-18       Impact factor: 3.240

Review 2.  Applications of Replicating-Competent Reporter-Expressing Viruses in Diagnostic and Molecular Virology.

Authors:  Yongfeng Li; Lian-Feng Li; Shaoxiong Yu; Xiao Wang; Lingkai Zhang; Jiahui Yu; Libao Xie; Weike Li; Razim Ali; Hua-Ji Qiu
Journal:  Viruses       Date:  2016-05-06       Impact factor: 5.048

3.  Development of a novel Newcastle disease virus (NDV) neutralization test based on recombinant NDV expressing enhanced green fluorescent protein.

Authors:  Ana Chumbe; Ray Izquierdo-Lara; Katherine Calderón; Manolo Fernández-Díaz; Vikram N Vakharia
Journal:  Virol J       Date:  2017-11-23       Impact factor: 4.099

4.  Characterization of a Cytopathogenic Reporter CSFV.

Authors:  Carina Maria Reuscher; Lisa Schmidt; Anette Netsch; Benjamin Lamp
Journal:  Viruses       Date:  2021-06-23       Impact factor: 5.048
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.