Jae-Sung Kwon1, Rasika P Illeperuma, Jin Kim, Kwang-Mahn Kim, Kyoung-Nam Kim. 1. Research Center for Orofacial Hard Tissue Regeneration and Department and Research Institute of Dental Biomaterials and Bioengineering, Yonsei University College of Dentistry , Seoul , Republic of Korea.
Abstract
OBJECTIVES: Despite being commonly used as temporary cements in dentistry, there is a lack of studies regarding the cytotoxicity of zinc oxide-eugenol (ZOE) and zinc oxide non-eugenol (ZONE) cements. In addition, cytotoxicity evaluation of the materials often involves animal-based cells. Therefore, in this study, a cytotoxicity evaluation of commercially available ZOE and ZONE cements was carried out using both animal and human-based cells. MATERIALS AND METHODS: The extraction or dilution of the extraction from four commercially available cements (two zinc oxide-eugenol and two zinc oxide non-eugenol) was tested for cytotoxicity, using three different cells and a water-soluble treatzolium salt assay. The results were confirmed using a confocal laser microscope following calcein AM and ethidium homodimer-1 staining. RESULTS: The results showed that there was a significant difference in cell viability depending on which cell was used, even when the same material was tested. Generally, L929 showed relatively low cell viability with a low EC50 (effective concentration of extracts that caused 50% of cell viability compared to the control) value compared to both HGF-1 and hTERT-hNOF. Such results were also confirmed by a confocal laser microscope. CONCLUSIONS: Careful consideration on interpreting the results for cytotoxicity evaluation of ZOE and ZONE cements is needed when different cells are used.
OBJECTIVES: Despite being commonly used as temporary cements in dentistry, there is a lack of studies regarding the cytotoxicity of zinc oxide-eugenol (ZOE) and zinc oxide non-eugenol (ZONE) cements. In addition, cytotoxicity evaluation of the materials often involves animal-based cells. Therefore, in this study, a cytotoxicity evaluation of commercially available ZOE and ZONE cements was carried out using both animal and human-based cells. MATERIALS AND METHODS: The extraction or dilution of the extraction from four commercially available cements (two zinc oxide-eugenol and two zinc oxide non-eugenol) was tested for cytotoxicity, using three different cells and a water-soluble treatzolium salt assay. The results were confirmed using a confocal laser microscope following calcein AM and ethidium homodimer-1 staining. RESULTS: The results showed that there was a significant difference in cell viability depending on which cell was used, even when the same material was tested. Generally, L929 showed relatively low cell viability with a low EC50 (effective concentration of extracts that caused 50% of cell viability compared to the control) value compared to both HGF-1 and hTERT-hNOF. Such results were also confirmed by a confocal laser microscope. CONCLUSIONS: Careful consideration on interpreting the results for cytotoxicity evaluation of ZOE and ZONE cements is needed when different cells are used.
Authors: Oana M Aburel; Ioana Z Pavel; Maria D Dănilă; Theia Lelcu; Alexandra Roi; Rodica Lighezan; Danina M Muntean; Laura C Rusu Journal: Oxid Med Cell Longev Date: 2021-03-02 Impact factor: 6.543