OBJECTIVES: In this study, we aimed to evaluate possible effects of leptin on fracture healing. MATERIALS AND METHODS: Between November 2009 and May 2010, 54 male Sprague Dawley rats with a mean age of 16 weeks and a mean body weight of 323 g (range; 295-352 g) were included and randomly divided into three equal groups. Standardized tibial fractures were created to all rats. Group 1 was treated with 0.5 mL physiological saline, group 2 was treated with 0.1 µg/kg leptin within 0.5 mL physiological saline, and group 3 was treated 0.3 µg/kg leptin within 0.5 mL physiological saline for 14 days intraperitoneally. Each group was divided into three subgroups including six rats for the evaluation at second, third and fifth weeks. RESULTS: Radiological evaluation showed that fracture healing of group 3 was better than group 1 (p=0.014) only at fifth week. Histological evaluation revealed fracture healing of group 3 was better than group 1 at third week (p=0.011). At fifth week, both group 2 and group 3 had better fracture healing than group 1 (p=0.006, p=0.002, respectively). There was no statistically significant difference in fracture healing between group 2 and 3 at fifth week (p=0.176). CONCLUSION: Leptin has a positive dose-dependent effect on rat tibial fracture healing.
OBJECTIVES: In this study, we aimed to evaluate possible effects of leptin on fracture healing. MATERIALS AND METHODS: Between November 2009 and May 2010, 54 male Sprague Dawley rats with a mean age of 16 weeks and a mean body weight of 323 g (range; 295-352 g) were included and randomly divided into three equal groups. Standardized tibial fractures were created to all rats. Group 1 was treated with 0.5 mL physiological saline, group 2 was treated with 0.1 µg/kg leptin within 0.5 mL physiological saline, and group 3 was treated 0.3 µg/kg leptin within 0.5 mL physiological saline for 14 days intraperitoneally. Each group was divided into three subgroups including six rats for the evaluation at second, third and fifth weeks. RESULTS: Radiological evaluation showed that fracture healing of group 3 was better than group 1 (p=0.014) only at fifth week. Histological evaluation revealed fracture healing of group 3 was better than group 1 at third week (p=0.011). At fifth week, both group 2 and group 3 had better fracture healing than group 1 (p=0.006, p=0.002, respectively). There was no statistically significant difference in fracture healing between group 2 and 3 at fifth week (p=0.176). CONCLUSION:Leptin has a positive dose-dependent effect on rat tibial fracture healing.
Authors: Mehmet Isyar; Seyit Ali Gumustas; Ibrahim Yilmaz; Duygu Yasar Sirin; Hacı Bayram Tosun; Mahir Mahirogullari Journal: Open Orthop J Date: 2016-09-09
Authors: F Graef; R Seemann; A Garbe; K Schmidt-Bleek; K D Schaser; J Keller; G Duda; S Tsitsilonis Journal: J Musculoskelet Neuronal Interact Date: 2017-06-01 Impact factor: 2.041