BACKGROUND: Minimal change disease (MCD) is the most common cause of nephrotic syndrome in children and is associated with the expression of CD80 in podocytes and the increased excretion of CD80 in urine. We hypothesized that serum from patients with MCD might stimulate CD80 expression in cultured podocytes. METHODS: Sera and peripheral blood mononuclear cells (PBMCs) were collected from subjects with MCD in relapse and remission and from normal controls. Immortalized human podocytes were incubated with culture media containing patient sera or supernatants from patient and control PBMC cultures. CD80 expression was measured by quantitative PCR and western blot analysis. RESULTS: Sera collected from patients with MCD in relapse, but not in remission, significantly increased CD80 expression (mean ± standard deviation: 1.8 ± 0.7 vs. 0.8 ± 0.2; p < 0.004) and CD80 protein secretion by podocytes (p < 0.05 between relapse and normal controls). No such CD80 increase was observed when podocytes were incubated with supernatants of PBMC cultures from patients in relapse. CONCLUSIONS: Sera from MCD patients in relapse, but not in remission, stimulated CD80 expression in cultured podocytes. Identifying this factor in sera could provide insights into the pathogenesis of this disorder. No role in CD80 expression by podocytes was found for cytokines released by PBMCs.
BACKGROUND: Minimal change disease (MCD) is the most common cause of nephrotic syndrome in children and is associated with the expression of CD80 in podocytes and the increased excretion of CD80 in urine. We hypothesized that serum from patients with MCD might stimulate CD80 expression in cultured podocytes. METHODS: Sera and peripheral blood mononuclear cells (PBMCs) were collected from subjects with MCD in relapse and remission and from normal controls. Immortalized human podocytes were incubated with culture media containing patient sera or supernatants from patient and control PBMC cultures. CD80 expression was measured by quantitative PCR and western blot analysis. RESULTS: Sera collected from patients with MCD in relapse, but not in remission, significantly increased CD80 expression (mean ± standard deviation: 1.8 ± 0.7 vs. 0.8 ± 0.2; p < 0.004) and CD80 protein secretion by podocytes (p < 0.05 between relapse and normal controls). No such CD80 increase was observed when podocytes were incubated with supernatants of PBMC cultures from patients in relapse. CONCLUSIONS: Sera from MCD patients in relapse, but not in remission, stimulated CD80 expression in cultured podocytes. Identifying this factor in sera could provide insights into the pathogenesis of this disorder. No role in CD80 expression by podocytes was found for cytokines released by PBMCs.
Authors: Maria Xatzipsalti; Serena Kyrana; Mariza Tsolia; Stelios Psarras; Apostolos Bossios; Vasile Laza-Stanca; Sebastian L Johnston; Nikolaos G Papadopoulos Journal: Am J Respir Crit Care Med Date: 2005-06-30 Impact factor: 21.405
Authors: Honghui Yu; Tuanzhu Ha; Li Liu; Xiaohui Wang; Ming Gao; Jim Kelley; Race Kao; David Williams; Chuanfu Li Journal: Biochim Biophys Acta Date: 2012-05-14
Authors: Eduardo H Garin; Wei Mu; John M Arthur; Christopher J Rivard; Carlos E Araya; Michiko Shimada; Richard J Johnson Journal: Kidney Int Date: 2010-05-19 Impact factor: 10.612
Authors: Kin-Wai Lai; Chang-Li Wei; Li-Kiang Tan; Puay-Hoon Tan; Gilbert S C Chiang; Caroline G L Lee; Stanley C Jordan; Hui-Kim Yap Journal: J Am Soc Nephrol Date: 2007-04-11 Impact factor: 10.121
Authors: Michiko Shimada; Takuji Ishimoto; Pui Y Lee; Miguel A Lanaspa; Christopher J Rivard; Carlos A Roncal-Jimenez; David T Wymer; Hideaki Yamabe; Peter W Mathieson; Moin A Saleem; Eduardo H Garin; Richard J Johnson Journal: Nephrol Dial Transplant Date: 2011-05-26 Impact factor: 5.992
Authors: Gabriel Cara-Fuentes; Miguel A Lanaspa; Gabriela E Garcia; Mindy Banks; Eduardo H Garin; Richard J Johnson Journal: Pediatr Nephrol Date: 2018-03-01 Impact factor: 3.714