| Literature DB >> 23684749 |
Alexandra E Gylfe1, Johanna Kondelin, Mikko Turunen, Heikki Ristolainen, Riku Katainen, Esa Pitkänen, Eevi Kaasinen, Ville Rantanen, Tomas Tanskanen, Markku Varjosalo, Heli Lehtonen, Kimmo Palin, Minna Taipale, Jussi Taipale, Laura Renkonen-Sinisalo, Heikki Järvinen, Jan Böhm, Jukka-Pekka Mecklin, Ari Ristimäki, Outi Kilpivaara, Sari Tuupanen, Auli Karhu, Pia Vahteristo, Lauri A Aaltonen.
Abstract
Microsatellite instability can be found in approximately 15% of all colorectal cancers. To detect new oncogenes we sequenced the exomes of 25 colorectal tumors and respective healthy colon tissue. Potential mutation hot spots were confirmed in 15 genes; ADAR, DCAF12L2, GLT1D1, ITGA7, MAP1B, MRGPRX4, PSRC1, RANBP2, RPS6KL1, SNCAIP, TCEAL6, TUBB6, WBP5, VEGFB, and ZBTB2; these were validated in 86 tumors with microsatellite instability. ZBTB2, RANBP2, and PSRC1 also were found to contain hot spot mutations in the validation set. The form of ZBTB2 associated with colorectal cancer increased cell proliferation. The mutation hot spots might be used to develop personalized tumor profiling and therapy.Entities:
Keywords: CRC; Exome Sequencing; Genetic Analysis; MSI; Mismatch Repair; PCR; colorectal cancer; microsatellite instability; polymerase chain reaction
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Year: 2013 PMID： 23684749 DOI： 10.1053/j.gastro.2013.05.015
Source DB: PubMed Journal: Gastroenterology ISSN： 0016-5085 Impact factor: 22.682