OBJECTIVE: To evaluate the relationship between transforming growth factor (TGF)-β1 and its receptor, soluble endoglin (sENG), in the serum and follicular fluid of women with polycystic ovarian syndrome (PCOS) compared with that of non-PCOS normal ovulating women during controlled ovarian stimulation (COS). DESIGN: Prospective case-control study. SETTING: Academic-affiliated assisted reproductive technology unit. PATIENT(S): Fourteen PCOS and 14 matched non-PCOS control women undergoing COS. INTERVENTION(S): Serum was collected on day 3 (baseline), day of hCG, and day of retrieval. Follicular fluid (FF) was collected on day of oocyte retrieval. ELISA was performed to determine TGF-β1 and sENG protein levels. MAIN OUTCOME MEASURE(S): Serum and FF levels of TGF-β1 and sENG. RESULT(S): Serum TGF-β1 did not change significantly during COS but was increased in PCOS compared with non-PCOS women on day 3 and days of hCG administration and oocyte retrieval. Serum sENG increased after hCG administration only in the non-PCOS control group. In addition, serum sENG was decreased in PCOS compared with non-PCOS control women on the days of hCG and retrieval. Accordingly, the bioavailability of TGF-β1 (TGF-β1/sENG ratio) was increased in women with PCOS compared with non-PCOS controls at all three time points. No differences in either factor were noted in FF between groups. CONCLUSION(S): The increased TGF-β1 bioavailability in PCOS is not only due to increased TGF-β1 levels but also to decreased levels of its receptor, sENG. These data suggest that increased TGF-β1 bioavailability may contribute to the pathogenesis of PCOS and its increased risk for ovarian hyperstimulation.
OBJECTIVE: To evaluate the relationship between transforming growth factor (TGF)-β1 and its receptor, soluble endoglin (sENG), in the serum and follicular fluid of women with polycystic ovarian syndrome (PCOS) compared with that of non-PCOS normal ovulating women during controlled ovarian stimulation (COS). DESIGN: Prospective case-control study. SETTING: Academic-affiliated assisted reproductive technology unit. PATIENT(S): Fourteen PCOS and 14 matched non-PCOS control women undergoing COS. INTERVENTION(S): Serum was collected on day 3 (baseline), day of hCG, and day of retrieval. Follicular fluid (FF) was collected on day of oocyte retrieval. ELISA was performed to determine TGF-β1 and sENG protein levels. MAIN OUTCOME MEASURE(S): Serum and FF levels of TGF-β1 and sENG. RESULT(S): Serum TGF-β1 did not change significantly during COS but was increased in PCOS compared with non-PCOSwomen on day 3 and days of hCG administration and oocyte retrieval. Serum sENG increased after hCG administration only in the non-PCOS control group. In addition, serum sENG was decreased in PCOS compared with non-PCOS control women on the days of hCG and retrieval. Accordingly, the bioavailability of TGF-β1 (TGF-β1/sENG ratio) was increased in women with PCOS compared with non-PCOS controls at all three time points. No differences in either factor were noted in FF between groups. CONCLUSION(S): The increased TGF-β1 bioavailability in PCOS is not only due to increased TGF-β1 levels but also to decreased levels of its receptor, sENG. These data suggest that increased TGF-β1 bioavailability may contribute to the pathogenesis of PCOS and its increased risk for ovarian hyperstimulation.
Authors: Eun Youn Roh; Jong Hyun Yoon; Eun Young Song; Jin Ju Kim; Kyu Ri Hwang; Soo Hyun Seo; Sue Shin Journal: J Assist Reprod Genet Date: 2016-10-29 Impact factor: 3.412
Authors: Luke C McIlvenna; Rhiannon K Patten; Andrew J McAinch; Raymond J Rodgers; Nigel K Stepto; Alba Moreno-Asso Journal: Front Endocrinol (Lausanne) Date: 2021-10-11 Impact factor: 5.555
Authors: Luke C McIlvenna; Ali Altıntaş; Rhiannon K Patten; Andrew J McAinch; Raymond J Rodgers; Nigel K Stepto; Romain Barrès; Alba Moreno-Asso Journal: J Physiol Date: 2022-06-27 Impact factor: 6.228