INTRODUCTION: CD4+CD25+ T lymphocytes have been implicated in the regulation of host inflammatory response against Trypanosoma cruzi, and may be involved in the clinical course of the disease. METHODS: Peripheral blood mononuclear cells from patients with chronic Chagas disease were cultured in the presence of T. cruzi recombinant antigens and assayed for lymphocytes at distinct time points. RESULTS: It was possible to differentiate clinical forms of chronic Chagas disease at days 3 and 5 according to presence of CD4+CD25+ T cells in cell cultures. CONCLUSIONS: Longer periods of cell culture proved to be potentially valuable for prospective evaluations of CD4+CD25+ T lymphocytes in patients with chronic Chagas disease.
INTRODUCTION:CD4+CD25+ T lymphocytes have been implicated in the regulation of host inflammatory response against Trypanosoma cruzi, and may be involved in the clinical course of the disease. METHODS: Peripheral blood mononuclear cells from patients with chronic Chagas disease were cultured in the presence of T. cruzi recombinant antigens and assayed for lymphocytes at distinct time points. RESULTS: It was possible to differentiate clinical forms of chronic Chagas disease at days 3 and 5 according to presence of CD4+CD25+ T cells in cell cultures. CONCLUSIONS: Longer periods of cell culture proved to be potentially valuable for prospective evaluations of CD4+CD25+ T lymphocytes in patients with chronic Chagas disease.
Authors: Suellen Carvalho de Moura Braz; Adriene Siqueira de Melo; Maria da Glória Aureliano de Melo Cavalcanti; Sílvia Marinho Martins; Wilson de Oliveira; Edimilson Domingos da Silva; Antonio Gomes Pinto Ferreira; Virginia Maria Barros de Lorena; Yara de Miranda Gomes Journal: J Clin Immunol Date: 2014-09-10 Impact factor: 8.317