Christoph Wiedemann1, Peter Bellstedt, Matthias Görlach. 1. Biomolecular NMR Spectroscopy, Leibniz Institute for Age Research-Fritz Lipmann Institute, Beutenbergstr. 11, 07745 Jena, Germany. cwiede@fli-leibniz.de
Abstract
MOTIVATION: Circular dichroism (CD) spectroscopy is one of the most versatile tools to study protein folding and to validate the proper fold of purified proteins. Here, we aim to provide a readily accessible, user-friendly and platform-independent tool capable of analysing multiple CD datasets of virtually any format and returning results as high-quality graphical output to the user. RESULTS: CAPITO (CD Anaylsis and Plotting Tool) is a novel web server-based tool for analysing and plotting CD data. It allows reliable estimation of secondary structure content utilizing different approaches. CAPITO accepts multiple CD datasets and, hence, is well suited for a wide application range such as the analysis of temperature or pH-dependent (un)folding and the comparison of mutants. AVAILABILITY: http://capito.nmr.fli-leibniz.de. CONTACT: cwiede@fli-leibniz.de or mago@fli-leibniz.de SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.
MOTIVATION: Circular dichroism (CD) spectroscopy is one of the most versatile tools to study protein folding and to validate the proper fold of purified proteins. Here, we aim to provide a readily accessible, user-friendly and platform-independent tool capable of analysing multiple CD datasets of virtually any format and returning results as high-quality graphical output to the user. RESULTS: CAPITO (CD Anaylsis and Plotting Tool) is a novel web server-based tool for analysing and plotting CD data. It allows reliable estimation of secondary structure content utilizing different approaches. CAPITO accepts multiple CD datasets and, hence, is well suited for a wide application range such as the analysis of temperature or pH-dependent (un)folding and the comparison of mutants. AVAILABILITY: http://capito.nmr.fli-leibniz.de. CONTACT: cwiede@fli-leibniz.de or mago@fli-leibniz.de SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.
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