Mohammad Pourhassan-Moghaddam1, Behrouz Farhadi, Kazem Nejati-Koshki, Tamouchin Moharrami. 1. Department of Medical Biotechnology, School of Advanced Biomedical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran ; Department of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran ; Students' Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
Abstract
INTRODUCTION: Production of complex human recombinant proteins is an important issue in medical biotechnology. These proteins are mostly expressed in non-human mammalian host cells. This has some problems including non-human post-translational modifications, application of high-cost agents for inducing protein expression and low yields. Thus, it is necessary to use new expression systems to overcome the indicated challenges. METHODS: In this paper, we hypothesize the application of promoter regions of cancer genes, which have a high rate of transcription in human cancer cell lines, for designing new expression vectors. RESULTS: After designing, these vectors could be applied to produce complex hu-man recombinant proteins in the human cancer cell lines as production hosts. CONCLUSION: Application of these expression vectors for the production of recombinant human proteins in the human cancer cell lines have some advantages including authentic post-translational modifications, proper-cost of commercialization, and high yields.
INTRODUCTION: Production of complex human recombinant proteins is an important issue in medical biotechnology. These proteins are mostly expressed in non-humanmammalian host cells. This has some problems including non-human post-translational modifications, application of high-cost agents for inducing protein expression and low yields. Thus, it is necessary to use new expression systems to overcome the indicated challenges. METHODS: In this paper, we hypothesize the application of promoter regions of cancer genes, which have a high rate of transcription in humancancer cell lines, for designing new expression vectors. RESULTS: After designing, these vectors could be applied to produce complex hu-man recombinant proteins in the humancancer cell lines as production hosts. CONCLUSION: Application of these expression vectors for the production of recombinant human proteins in the humancancer cell lines have some advantages including authentic post-translational modifications, proper-cost of commercialization, and high yields.
Entities:
Keywords:
Cancer Genes; Human Cancer Cell Lines; Promoters; Recombinant Protein Expression