Literature DB >> 236771

Isolation of an extracellular neutral proteinase from Pseudomonas fragi.

M A Porzio, A M Pearson.   

Abstract

A single proteolytic enzyme (EC 3.4.4.-) was isolated from culture supernatants of Pseudomonas fragi with 20% yielded and 60-fold purification by means of stepwise DEAE-Sephadex batch adsorption, ammonium sulfate precipitation, gel filtration and DEAE-cellulose chromatography. The enzyme was Zn-2+ activated and Ca-2+ stabilized, had optimum activity at pH 6.5--8.0 and 40 degrees C. The molecular weight range was 40 000--50 000 as determined by dodecylsulfate gel electrophoresis, gel filtration and Zn assay. This proteinase has properties similar to other extracellular bacterial neutral proteinases.

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Year:  1975        PMID： 236771     DOI： 10.1016/0005-2744(75)90112-6

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  6 in total

1.  The extracellular protease from Pseudomonas fluorescens.

Authors:  S M Juan; J J Cazzulo
Journal:  Experientia       Date:  1976-09-15

Review 2.  Bacterial extracellular zinc-containing metalloproteases.

Authors:  C C Häse; R A Finkelstein
Journal:  Microbiol Rev       Date:  1993-12

3.  Stabilization of a psychrotrophic Pseudomonas protease by calcium against thermal inactivation in milk at ultrahigh temperature.

Authors:  J T Barach; D M Adams; M L Speck
Journal:  Appl Environ Microbiol       Date:  1976-06       Impact factor: 4.792

4.  Isolation and characterization of proteases from Bacteroides melaninogenicus.

Authors:  S Fujimura; T Nakamura
Journal:  Infect Immun       Date:  1981-09       Impact factor: 3.441

5.  Ultrastructural localization of an extracellular protease in Pseudomonas fragi by using the peroxidase-antiperoxidase reaction.

Authors:  S S Thompson; Y M Naidu; J J Pestka
Journal:  Appl Environ Microbiol       Date:  1985-10       Impact factor: 4.792

6.  Isolation and properties of the protease from the wild-type and mutant strains of Pseudomonas fragi.

Authors:  J Noreau; G R Drapeau
Journal:  J Bacteriol       Date:  1979-12       Impact factor: 3.490
  6 in total

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