CONTEXT: Thyroid carcinoma is one of the most common malignancies of the endocrine system, and, despite the high frequency of oncogene activation in thyroid neoplastic lesions, the tumor suppressor genes involved in thyroid carcinogenesis remain unidentified. Our previous data implicated a link between the CL2/CCDC80 gene and thyroid cancer. OBJECTIVE: The objective of the study was to examine the expression of the CL2/CCDC80 gene in human thyroid carcinomas in the attempt to determine whether it plays a role in thyroid carcinogenesis. DESIGN: We evaluated the expression of CL2/CCDC80 in a large number of thyroid neoplastic tissue samples differing in degree of malignancy. We also investigated the effects of its restoration in 2 human thyroid carcinoma cell lines characterized by very low levels of CL2/CCDC80 expression. RESULTS: CL2/CCDC80 expression was much lower in almost all the thyroid carcinomas analyzed than in normal thyroid tissues and was lowest in follicular variants of papillary carcinomas. Loss of heterozygosity partially accounted for CL2/CCDC80 down-regulation in thyroid carcinoma samples. Restoration of CL2/CCDC80 expression in the 2 human thyroid anaplastic carcinoma cell lines resulted in a higher susceptibility to apoptosis and suppression of the malignant phenotype. CL2/CCDC80 expression positively regulated the expression of E-cadherin, thereby halting cancer progression. CONCLUSIONS: These results indicate that CL2/CCDC80 is a putative tumor suppressor gene in thyroid carcinogenesis.
CONTEXT: Thyroid carcinoma is one of the most common malignancies of the endocrine system, and, despite the high frequency of oncogene activation in thyroid neoplastic lesions, the tumor suppressor genes involved in thyroid carcinogenesis remain unidentified. Our previous data implicated a link between the CL2/CCDC80 gene and thyroid cancer. OBJECTIVE: The objective of the study was to examine the expression of the CL2/CCDC80 gene in humanthyroid carcinomas in the attempt to determine whether it plays a role in thyroid carcinogenesis. DESIGN: We evaluated the expression of CL2/CCDC80 in a large number of thyroid neoplastic tissue samples differing in degree of malignancy. We also investigated the effects of its restoration in 2 humanthyroid carcinoma cell lines characterized by very low levels of CL2/CCDC80 expression. RESULTS:CL2/CCDC80 expression was much lower in almost all the thyroid carcinomas analyzed than in normal thyroid tissues and was lowest in follicular variants of papillary carcinomas. Loss of heterozygosity partially accounted for CL2/CCDC80 down-regulation in thyroid carcinoma samples. Restoration of CL2/CCDC80 expression in the 2 humanthyroid anaplastic carcinoma cell lines resulted in a higher susceptibility to apoptosis and suppression of the malignant phenotype. CL2/CCDC80 expression positively regulated the expression of E-cadherin, thereby halting cancer progression. CONCLUSIONS: These results indicate that CL2/CCDC80 is a putative tumor suppressor gene in thyroid carcinogenesis.
Authors: Trillitye Paullin; Chase Powell; Christopher Menzie; Robert Hill; Feng Cheng; Christopher J Martyniuk; Sandy D Westerheide Journal: PLoS One Date: 2017-08-09 Impact factor: 3.240
Authors: Sirui Zhou; Ziv Gan-Or; Amirthagowri Ambalavanan; Dongbing Lai; Pingxing Xie; Cynthia V Bourassa; Stephanie Strong; Jay P Ross; Alexandre Dionne-Laporte; Dan Spiegelman; Nicolas Dupré; Tatiana M Foroud; Lan Xiong; Patrick A Dion; Guy A Rouleau Journal: Sci Rep Date: 2018-03-12 Impact factor: 4.379
Authors: Sharadindu Shil; R S Joshi; C G Joshi; A K Patel; Ravi K Shah; Namrata Patel; Subhash J Jakhesara; Sumana Kundu; Bhaskar Reddy; P G Koringa; D N Rank Journal: Vet World Date: 2017-01-13