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Literature DB >> 23664906

Transposon mutagenesis of Bacteroides fragilis using a mariner transposon vector.

Yaligara Veeranagouda¹, Fasahath Husain, Hannah M Wexler.

Abstract

The mariner transposon vector pYV07 was tested for use in the mutagenesis of Bacteroides fragilis 638R. The transposon vector efficiently generated mutants in B. fragilis 638R. The transposon disrupted genes were scattered throughout the genome of B. fragilis 638R. This method serves as a powerful tool to study B. fragilis. Published by Elsevier Ltd.

Entities: Chemical Disease Species

Keywords: Bacteroides fragilis; Mariner transposon; Mutant library; Rescue cloning; Semi random PCR; Transposon mutagenesis

Mesh：

Substances：

Year: 2013 PMID： 23664906 PMCID： PMC3729920 DOI： 10.1016/j.anaerobe.2013.04.012

Source DB: PubMed Journal: Anaerobe ISSN： 1075-9964 Impact factor: 3.331

19 in total

1. Mobile genetic elements in the genus Bacteroides, and their mechanism(s) of dissemination.

Authors: Mai Nguyen; Gayatri Vedantam
Journal: Mob Genet Elements Date: 2011-09-01

2. Hyperactive transposase mutants of the Himar1 mariner transposon.

Authors: D J Lampe; B J Akerley; E J Rubin; J J Mekalanos; H M Robertson
Journal: Proc Natl Acad Sci U S A Date: 1999-09-28 Impact factor: 11.205

Review 3. The role of anaerobic bacteria in bacteremia.

Authors: Itzhak Brook
Journal: Anaerobe Date: 2009-12-16 Impact factor: 3.331

4. Transposon mutagenesis of the anaerobic commensal, Bacteroides fragilis, using the EZ::TN5 transposome.

Authors: Yaligara Veeranagouda; Fasahath Husain; Hannah M Wexler
Journal: FEMS Microbiol Lett Date: 2012-06-18 Impact factor: 2.742

5. Application of quantitative real-time PCR for rapid identification of Bacteroides fragilis group and related organisms in human wound samples.

Authors: Jia Tong; Chengxu Liu; Paula Summanen; Huaxi Xu; Sydney M Finegold
Journal: Anaerobe Date: 2011-03-23 Impact factor: 3.331

Review 6. Bacteroides spp. and diarrhea.

Authors: Elizabeth C Wick; Cynthia L Sears
Journal: Curr Opin Infect Dis Date: 2010-10 Impact factor: 4.915

7. Human fecal flora: the normal flora of 20 Japanese-Hawaiians.

Authors: W E Moore; L V Holdeman
Journal: Appl Microbiol Date: 1974-05

8. Microbial etiology of travelers' diarrhea in Mexico, Guatemala, and India: importance of enterotoxigenic Bacteroides fragilis and Arcobacter species.

Authors: Zhi-Dong Jiang; Herbert L Dupont; Eric L Brown; Ranjan K Nandy; Thandavaryan Ramamurthy; Anuradha Sinha; Santanu Ghosh; Sucharita Guin; Kaur Gurleen; Savio Rodrigues; Jacklyn J Chen; Robin McKenzie; Robert Steffen
Journal: J Clin Microbiol Date: 2010-01-27 Impact factor: 5.948

4. Identification of genes required for the survival of B. fragilis using massive parallel sequencing of a saturated transposon mutant library.

Authors: Yaligara Veeranagouda; Fasahath Husain; Elizabeth L Tenorio; Hannah M Wexler
Journal: BMC Genomics Date: 2014-06-04 Impact factor: 3.969

4 in total

Transposon mutagenesis of Bacteroides fragilis using a mariner transposon vector.

1. Mobile genetic elements in the genus Bacteroides, and their mechanism(s) of dissemination.

2. Hyperactive transposase mutants of the Himar1 mariner transposon.

Review 3. The role of anaerobic bacteria in bacteremia.

4. Transposon mutagenesis of the anaerobic commensal, Bacteroides fragilis, using the EZ::TN5 transposome.

5. Application of quantitative real-time PCR for rapid identification of Bacteroides fragilis group and related organisms in human wound samples.

Review 6. Bacteroides spp. and diarrhea.

7. Human fecal flora: the normal flora of 20 Japanese-Hawaiians.

8. Microbial etiology of travelers' diarrhea in Mexico, Guatemala, and India: importance of enterotoxigenic Bacteroides fragilis and Arcobacter species.

9. Genome-scale identification of resistance functions in Pseudomonas aeruginosa using Tn-seq.

10. Identification of essential genes of the periodontal pathogen Porphyromonas gingivalis.

1. Deficiency of the ferrous iron transporter FeoAB is linked with metronidazole resistance in Bacteroides fragilis.

2. Elizabethkingia anophelis: molecular manipulation and interactions with mosquito hosts.

3. The Application of Transposon Insertion Sequencing in Identifying Essential Genes in B. fragilis.

4. Identification of genes required for the survival of B. fragilis using massive parallel sequencing of a saturated transposon mutant library.