BACKGROUND: The persistence of HIV residual replication in patients with an undetectable plasma viral load (pVL) may limit immune recovery and facilitate inflammation-induced comorbidities. OBJECTIVE: The objective was to evaluate any correlation between immune restoration and intracellular [IC] HIV-DNA in cART-treated patients with a sustained undetectable pVL. STUDY DESIGN: This retrospective cross sectional study included 62 patients with a median duration of undetectable pVL of 10.3 years. IC HIV DNA in peripheral mononuclear blood cells (PBMCs) and T cell subsets were measured at the last visit. pVL, CD4(+) and CD8(+) T cell counts were retrospectively collected from the onset of long-term inhibition by antiretroviral treatment. The patients were separated into two groups: 27 non-blippers (sustained pVL< threshold value during all the visits) and 35 blippers ( ≥ 1 episodes of pVL> threshold but < 1000 copies/ml). The median pVL in blippers was 115 copies/ml. RESULTS: The median IC HIV DNA rate was 34 copies/10(6) PBMCs (71% ≥ 20 copies/10(6) PBMCs) with no significant difference between the groups. The proportion of CD8(+)CD38(+) and CD8(+)DR(+) T cells was higher in blipper patients, but the difference was only significant for the CD8(+)DR(+) marker (p = 0.036). No correlation was found between markers of immune activation on CD4(+) and CD8(+) T cells and the IC HIV-DNA level. CONCLUSION: No relation was found between the size of HIV reservoirs and immune activation in patients with sustained undetectable pVL. Mechanisms of immune activation have to be better understood in order to define specific therapeutic interventions.
BACKGROUND: The persistence of HIV residual replication in patients with an undetectable plasma viral load (pVL) may limit immune recovery and facilitate inflammation-induced comorbidities. OBJECTIVE: The objective was to evaluate any correlation between immune restoration and intracellular [IC] HIV-DNA in cART-treated patients with a sustained undetectable pVL. STUDY DESIGN: This retrospective cross sectional study included 62 patients with a median duration of undetectable pVL of 10.3 years. IC HIV DNA in peripheral mononuclear blood cells (PBMCs) and T cell subsets were measured at the last visit. pVL, CD4(+) and CD8(+) T cell counts were retrospectively collected from the onset of long-term inhibition by antiretroviral treatment. The patients were separated into two groups: 27 non-blippers (sustained pVL< threshold value during all the visits) and 35 blippers ( ≥ 1 episodes of pVL> threshold but < 1000 copies/ml). The median pVL in blippers was 115 copies/ml. RESULTS: The median IC HIV DNA rate was 34 copies/10(6) PBMCs (71% ≥ 20 copies/10(6) PBMCs) with no significant difference between the groups. The proportion of CD8(+)CD38(+) and CD8(+)DR(+) T cells was higher in blipper patients, but the difference was only significant for the CD8(+)DR(+) marker (p = 0.036). No correlation was found between markers of immune activation on CD4(+) and CD8(+) T cells and the IC HIV-DNA level. CONCLUSION: No relation was found between the size of HIV reservoirs and immune activation in patients with sustained undetectable pVL. Mechanisms of immune activation have to be better understood in order to define specific therapeutic interventions.
Authors: Jesper F Højen; Thomas A Rasmussen; Karen Lise D Andersen; Anni A Winckelmann; Rune R Laursen; Jesper D Gunst; Holger J Møller; Mayumi Fujita; Lars Østergaard; Ole S Søgaard; Charles A Dinarello; Martin Tolstrup Journal: Mol Med Date: 2015-04-14 Impact factor: 6.354
Authors: Shane D Falcinelli; Bonnie E Shook-Sa; Morgan G Dewey; Sumati Sridhar; Jenna Read; Jennifer Kirchherr; Katherine S James; Brigitte Allard; Simon Ghofrani; Erin Stuelke; Caroline Baker; Nadia R Roan; Joseph J Eron; JoAnn D Kuruc; Catalina Ramirez; Cynthia Gay; Katie R Mollan; David M Margolis; Adaora A Adimora; Nancie M Archin Journal: J Infect Dis Date: 2020-11-09 Impact factor: 5.226
Authors: Gabriela Khoury; Rémi Fromentin; Ajantha Solomon; Wendy Hartogensis; Marisela Killian; Rebecca Hoh; Ma Somsouk; Peter W Hunt; Valerie Girling; Elizabeth Sinclair; Peter Bacchetti; Jenny L Anderson; Frederick M Hecht; Steven G Deeks; Paul U Cameron; Nicolas Chomont; Sharon R Lewin Journal: J Infect Dis Date: 2017-03-15 Impact factor: 5.226
Authors: Guillaume J Besson; Christina M Lalama; Ronald J Bosch; Rajesh T Gandhi; Margaret A Bedison; Evgenia Aga; Sharon A Riddler; Deborah K McMahon; Feiyu Hong; John W Mellors Journal: Clin Infect Dis Date: 2014-07-29 Impact factor: 9.079
Authors: F De Salvador-Guillouët; C Sakarovitch; J Durant; K Risso; E Demonchy; P M Roger; E Fontas Journal: PLoS One Date: 2015-10-20 Impact factor: 3.240
Authors: Leslie R Cockerham; Janet D Siliciano; Elizabeth Sinclair; Una O'Doherty; Sarah Palmer; Steven A Yukl; Matt C Strain; Nicolas Chomont; Frederick M Hecht; Robert F Siliciano; Douglas D Richman; Steven G Deeks Journal: PLoS One Date: 2014-10-23 Impact factor: 3.240