BACKGROUND AND OBJECTIVES: Autophagy is a self-degradation mechanism induced under stress conditions in all eukaryotic cells. Its activity in human lymphomas has not been studied as yet. METHODS: In this study, the autophagic activity of lymphoid cells was investigated in follicular lymphomas (FL; 48 cases), diffuse large B-cell lymphomas (DLBCL; 78 cases), and in reactive follicular hyperplasias (41 cases), using the light chain 3A (LC3A) antibody and a standard immunohistochemical technique. RESULTS: In all cases, the pattern of LC3A reactivity was uniformly diffuse cytoplasmic, but expressed more frequently in FLs (68.8%) than in DLBCLs (41%) (p=0.02), and much more commonly in DLBCLs than in reactive lymph nodes (24.3%) (p<0.006). Interestingly, FLs expressing LC3A in >10% of lymphoid cells (high reactivity) were associated with the hypoxia-related protein HIF1α and the enzyme of anaerobic metabolism lactate dehydrogenase LDH5 (p=0.004 and p=0.003, respectively). Such associations, however, were not a feature in DLBCLs of increased LC3A activity. CONCLUSIONS: LC3A expression in FLs is hypoxia-induced, whereas its expression in DLBCLs may be regulated by other molecular mechanisms. The current study provides a tool for further assessment of autophagic activity in translational and autophagy targeting therapy studies.
BACKGROUND AND OBJECTIVES: Autophagy is a self-degradation mechanism induced under stress conditions in all eukaryotic cells. Its activity in humanlymphomas has not been studied as yet. METHODS: In this study, the autophagic activity of lymphoid cells was investigated in follicular lymphomas (FL; 48 cases), diffuse large B-cell lymphomas (DLBCL; 78 cases), and in reactive follicular hyperplasias (41 cases), using the light chain 3A (LC3A) antibody and a standard immunohistochemical technique. RESULTS: In all cases, the pattern of LC3A reactivity was uniformly diffuse cytoplasmic, but expressed more frequently in FLs (68.8%) than in DLBCLs (41%) (p=0.02), and much more commonly in DLBCLs than in reactive lymph nodes (24.3%) (p<0.006). Interestingly, FLs expressing LC3A in >10% of lymphoid cells (high reactivity) were associated with the hypoxia-related protein HIF1α and the enzyme of anaerobic metabolism lactate dehydrogenase LDH5 (p=0.004 and p=0.003, respectively). Such associations, however, were not a feature in DLBCLs of increased LC3A activity. CONCLUSIONS:LC3A expression in FLs is hypoxia-induced, whereas its expression in DLBCLs may be regulated by other molecular mechanisms. The current study provides a tool for further assessment of autophagic activity in translational and autophagy targeting therapy studies.
Authors: P Oikonomou; A Giatromanolaki; A K Tsaroucha; K Balaska; C H Tsalikidis; C H Nikolaou; M Pitiakoudis; C Simopoulos Journal: Hippokratia Date: 2019 Apr-Jun Impact factor: 0.471
Authors: Xiu Li Feng; Man Man Zong; Guang Fang Zhou; Yang Zheng; Yuan Nan Yu; Rui Bing Cao; Pu Yan Chen; Mei Yang Journal: J Immunol Res Date: 2019-01-06 Impact factor: 4.818
Authors: M I Koukourakis; A Giatromanolaki; A Bottini; M R Cappelletti; L Zanotti; G Allevi; C Strina; M Ardine; M Milani; G Brugnoli; M Martinotti; G Ferrero; R Bertoni; F Ferrozzi; A L Harris; D Generali Journal: Br J Cancer Date: 2014-04-10 Impact factor: 7.640