| Literature DB >> 23661761 |
Ivelin S Georgiev1, Nicole A Doria-Rose, Tongqing Zhou, Young Do Kwon, Ryan P Staupe, Stephanie Moquin, Gwo-Yu Chuang, Mark K Louder, Stephen D Schmidt, Han R Altae-Tran, Robert T Bailer, Krisha McKee, Martha Nason, Sijy O'Dell, Gilad Ofek, Marie Pancera, Sanjay Srivatsan, Lawrence Shapiro, Mark Connors, Stephen A Migueles, Lynn Morris, Yoshiaki Nishimura, Malcolm A Martin, John R Mascola, Peter D Kwong.
Abstract
Serum characterization and antibody isolation are transforming our understanding of the humoral immune response to viral infection. Here, we show that epitope specificities of HIV-1-neutralizing antibodies in serum can be elucidated from the serum pattern of neutralization against a diverse panel of HIV-1 isolates. We determined "neutralization fingerprints" for 30 neutralizing antibodies on a panel of 34 diverse HIV-1 strains and showed that similarity in neutralization fingerprint correlated with similarity in epitope. We used these fingerprints to delineate specificities of polyclonal sera from 24 HIV-1-infected donors and a chimeric siman-human immunodeficiency virus-infected macaque. Delineated specificities matched published specificities and were further confirmed by antibody isolation for two sera. Patterns of virus-isolate neutralization can thus afford a detailed epitope-specific understanding of neutralizing-antibody responses to viral infection.Entities:
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Year: 2013 PMID： 23661761 DOI： 10.1126/science.1233989
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728