BACKGROUND: Molecular biomarkers are needed for diagnostic use in periodontal diseases. The aim of this study is to explore different gingival crevicular fluid (GCF) matrix metalloproteinase-8 (MMP-8) patterns in smokers and non-smokers with chronic periodontitis (CP) and test the utility of baseline GCF MMP-8 levels in predicting categorically assessed treatment outcomes. METHODS: The study population comprised 15 patients with CP (five non-smokers and 10 smokers). GCF sampling of five to seven periodontal sites per patient was done at baseline, post-treatment, and bimonthly during the maintenance period from 8 to 12 months. GCF MMP-8 levels were measured with an immunofluorometric assay. MMP-8 response patterns were explored by cluster analysis. The ability of baseline MMP-8 levels to predict categorical treatment outcomes was analyzed with receiver operating characteristic curves. RESULTS: GCF MMP-8 response patterns could be clustered into two different site profiles among both smokers and non-smokers. Smoker site profiles 1 and 2 had significantly different clinical attachment level and gingival recession changes by the end of the maintenance period. In smoker sites, baseline MMP-8 levels significantly predicted the categorical treatment outcome. CONCLUSIONS: Baseline GCF MMP-8 levels strongly predict how MMP-8 levels behave during the maintenance period. In smoker sites, high baseline MMP-8 levels indicate weak treatment response.
BACKGROUND: Molecular biomarkers are needed for diagnostic use in periodontal diseases. The aim of this study is to explore different gingival crevicular fluid (GCF) matrix metalloproteinase-8 (MMP-8) patterns in smokers and non-smokers with chronic periodontitis (CP) and test the utility of baseline GCF MMP-8 levels in predicting categorically assessed treatment outcomes. METHODS: The study population comprised 15 patients with CP (five non-smokers and 10 smokers). GCF sampling of five to seven periodontal sites per patient was done at baseline, post-treatment, and bimonthly during the maintenance period from 8 to 12 months. GCF MMP-8 levels were measured with an immunofluorometric assay. MMP-8 response patterns were explored by cluster analysis. The ability of baseline MMP-8 levels to predict categorical treatment outcomes was analyzed with receiver operating characteristic curves. RESULTS: GCF MMP-8 response patterns could be clustered into two different site profiles among both smokers and non-smokers. Smoker site profiles 1 and 2 had significantly different clinical attachment level and gingival recession changes by the end of the maintenance period. In smoker sites, baseline MMP-8 levels significantly predicted the categorical treatment outcome. CONCLUSIONS: Baseline GCF MMP-8 levels strongly predict how MMP-8 levels behave during the maintenance period. In smoker sites, high baseline MMP-8 levels indicate weak treatment response.
Authors: Saeed Alassiri; Pirjo Parnanen; Nilminie Rathnayake; Gunnar Johannsen; Anna-Maria Heikkinen; Richard Lazzara; Peter van der Schoor; Jan Gerrit van der Schoor; Taina Tervahartiala; Dirk Gieselmann; Timo Sorsa Journal: Dis Markers Date: 2018-08-05 Impact factor: 3.434
Authors: Timo Sorsa; Saeed Alassiri; Andreas Grigoriadis; Ismo T Räisänen; Pirjo Pärnänen; Solomon O Nwhator; Dirk-Rolf Gieselmann; Dimitra Sakellari Journal: Diagnostics (Basel) Date: 2020-01-22
Authors: Sarhang Sarwat Gul; Faraedon Mostafa Zardawi; Ali Abbas Abdulkareem; Muhammad Saad Shaikh; Natheer Hashim Al-Rawi; Muhammad Sohail Zafar Journal: Int J Environ Res Public Health Date: 2022-03-07 Impact factor: 4.614