OBJECTIVES: Tenascin-C plays an important role in myocardial and vascular remodelling. We hypothesized that tenascin-C is a key factor in the development of degenerative disease of the ascending aorta, leading to chronic dilatation and acute aortic dissection. METHODS: Ascending aortic wall specimens were obtained during surgery for chronic dilatation (n=52) and acute Type A dissection (n=30). Patients (n=12) undergoing aortic valve replacement served as controls. Tenascin-C expression was evaluated by immunostaining and semi-quantitatively assessed using the ImageJ software. TN-C levels in peripheral blood were determined by enzyme-linked immunosorbent assay. RESULTS: Histological examination showed a clear difference between chronic dilatation and acute dissection. In chronic dilatation, tenascin-C staining was homogenously distributed throughout the media parallel to vascular smooth muscle cells. In acute dissection, a strong staining with a heterogenous and spotty distribution was detected. Control aortas showed no tenascin-C staining. Tenascin-C expression was significantly higher in Type-A dissection compared with chronic dilatation. This was accompanied by a significant elevation of tenascin-C levels in peripheral blood in acute dissection. There was no statistical correlation between the tenascin-C level in peripheral blood and the aortic diameter either in dissection or in dilatation. CONCLUSIONS: Tenascin-C is a marker of progressive destabilization of the aortic wall independent of size in chronic dilatation and acute dissection. Therefore, it might be a valuable tool in guiding intervention strategies in patients with disease of the ascending aorta.
OBJECTIVES:Tenascin-C plays an important role in myocardial and vascular remodelling. We hypothesized that tenascin-C is a key factor in the development of degenerative disease of the ascending aorta, leading to chronic dilatation and acute aortic dissection. METHODS: Ascending aortic wall specimens were obtained during surgery for chronic dilatation (n=52) and acute Type A dissection (n=30). Patients (n=12) undergoing aortic valve replacement served as controls. Tenascin-C expression was evaluated by immunostaining and semi-quantitatively assessed using the ImageJ software. TN-C levels in peripheral blood were determined by enzyme-linked immunosorbent assay. RESULTS: Histological examination showed a clear difference between chronic dilatation and acute dissection. In chronic dilatation, tenascin-C staining was homogenously distributed throughout the media parallel to vascular smooth muscle cells. In acute dissection, a strong staining with a heterogenous and spotty distribution was detected. Control aortas showed no tenascin-C staining. Tenascin-C expression was significantly higher in Type-A dissection compared with chronic dilatation. This was accompanied by a significant elevation of tenascin-C levels in peripheral blood in acute dissection. There was no statistical correlation between the tenascin-C level in peripheral blood and the aortic diameter either in dissection or in dilatation. CONCLUSIONS:Tenascin-C is a marker of progressive destabilization of the aortic wall independent of size in chronic dilatation and acute dissection. Therefore, it might be a valuable tool in guiding intervention strategies in patients with disease of the ascending aorta.
Authors: E J Mackie; T Scott-Burden; A W Hahn; F Kern; J Bernhardt; S Regenass; A Weller; F R Bühler Journal: Am J Pathol Date: 1992-08 Impact factor: 4.307
Authors: Sara N Koenig; Stephanie LaHaye; James D Feller; Patrick Rowland; Kan N Hor; Aaron J Trask; Paul Ml Janssen; Freddy Radtke; Brenda Lilly; Vidu Garg Journal: JCI Insight Date: 2017-11-02
Authors: Hisashi Sawada; Satoko Ohno-Urabe; Dien Ye; Michael K Franklin; Jessica J Moorleghen; Deborah A Howatt; Adam E Mullick; Alan Daugherty; Hong S Lu Journal: Arterioscler Thromb Vasc Biol Date: 2022-08-25 Impact factor: 10.514
Authors: Felix Nagel; Anne-Kristin Schaefer; Inês Fonseca Gonçalves; Eylem Acar; Andre Oszwald; Philipp Kaiser; Renate Kain; Karola Trescher; Wolf H Eilenberg; Christine Brostjan; David Santer; Attila Kiss; Bruno K Podesser Journal: Interact Cardiovasc Thorac Surg Date: 2022-05-02