BACKGROUND: Tissue factor (TF), the main activator of blood coagulation, is expressed on platelet surface and, together with procoagulant phospholipids, contributes to the global coagulation potential of these blood components. The present study evaluated, for the first time, the expression of TF on platelet surface during preparation and storage of platelet concentrates (PC) for transfusional use. METHODS: Platelet TF was measured by flow cytometry in healthy donor whole blood (WB) and in pooled buffy-coat-derived PC on the day of preparation and up to 4 days of storage in parallel with classical markers of platelet activation, i.e., fibrinogen, P-selectin, and glycoprotein GPIIb. Data were analyzed according to donor age and blood ABO group. RESULTS: TF was detected on whole blood platelets and was found highest in O donors. Compared to whole blood, platelet surface TF was higher upon PC preparation and further increased during storage. The rise in TF levels positively correlated with the elevations of the other platelet markers. CONCLUSIONS: Our findings show that platelet surface TF is maintained in PC obtained by the pooled buffy coat method. Further studies are warranted to investigate a possible correlation between TF levels and the hemostatic response of the platelet transfusion recipient.
BACKGROUND:Tissue factor (TF), the main activator of blood coagulation, is expressed on platelet surface and, together with procoagulant phospholipids, contributes to the global coagulation potential of these blood components. The present study evaluated, for the first time, the expression of TF on platelet surface during preparation and storage of platelet concentrates (PC) for transfusional use. METHODS: Platelet TF was measured by flow cytometry in healthy donor whole blood (WB) and in pooled buffy-coat-derived PC on the day of preparation and up to 4 days of storage in parallel with classical markers of platelet activation, i.e., fibrinogen, P-selectin, and glycoprotein GPIIb. Data were analyzed according to donor age and blood ABO group. RESULTS:TF was detected on whole blood platelets and was found highest in O donors. Compared to whole blood, platelet surface TF was higher upon PC preparation and further increased during storage. The rise in TF levels positively correlated with the elevations of the other platelet markers. CONCLUSIONS: Our findings show that platelet surface TF is maintained in PC obtained by the pooled buffy coat method. Further studies are warranted to investigate a possible correlation between TF levels and the hemostatic response of the platelet transfusion recipient.
Authors: P Metcalfe; L M Williamson; C P Reutelingsperger; I Swann; W H Ouwehand; A H Goodall Journal: Br J Haematol Date: 1997-07 Impact factor: 6.998
Authors: A Zillmann; T Luther; I Müller; M Kotzsch; M Spannagl; T Kauke; U Oelschlägel; S Zahler; B Engelmann Journal: Biochem Biophys Res Commun Date: 2001-02-23 Impact factor: 3.575
Authors: Irene Lopez-Vilchez; Ana Maria Galan; Maria Rosa Hernandez; Carolina Caballo; Merce Roque; Maribel Diaz-Ricart; James G White; Gines Escolar Journal: Thromb Res Date: 2012-11-01 Impact factor: 3.944
Authors: M Shima; Y Fujimura; T Nishiyama; T Tsujiuchi; N Narita; T Matsui; K Titani; M Katayama; F Yamamoto; A Yoshioka Journal: Vox Sang Date: 1995 Impact factor: 2.144
Authors: Morris Madzime; Theresa M Rossouw; Annette J Theron; Ronald Anderson; Helen C Steel Journal: Front Immunol Date: 2021-03-12 Impact factor: 7.561