| Literature DB >> 23651157 |
Eric A Davidson1, Adam J Meyer, Jared W Ellefson, Matthew Levy, Andrew D Ellington.
Abstract
Recent technological advances have allowed development of increasingly complex systems for in vitro evolution. Here, we describe an in vitro autogene composed of a self-amplifying T7 RNA polymerase system. Functional autogene templates in cell-free lysate produce T7 RNA polymerase, which amplifies the autogene genetic information through a positive feedback architecture. Compartmentalization of individual templates within a water-in-oil emulsion links genotype and phenotype, allowing evolution.Entities:
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Year: 2012 PMID： 23651157 DOI： 10.1021/sb3000113
Source DB: PubMed Journal: ACS Synth Biol ISSN： 2161-5063 Impact factor: 5.110