BACKGROUND/AIM: We studied the effects of CO(2) concentration changes on the invasive ability of colon cancer cells. MATERIALS AND METHODS: Colon cancer cell lines and human samples derived from a peritoneal metastasis were incubated in a hypercapnic environment, followed by incubation in 5% CO(2). The invasive ability of colon cancer cells incubated with CO(2) were analyzed using an invasion assay system. RESULTS: In comparison with the colon cancer cell lines incubated in 5% CO(2) only, the invasive ability of cells increased in all the colon cancer cell lines subjected to incubation in 20% CO(2) followed by incubation in 5% CO(2), with a concomitant increase in the mRNA expressions of matrix metalloproteinase-2 (MMP2) and MMP9. The invasive capability of peritoneal metastatic cells in the human-derived specimen also increased on CO(2) concentration changes. CONCLUSION: CO(2) concentration changes enhanced the invasive capacity of colon cancer cells.
BACKGROUND/AIM: We studied the effects of CO(2) concentration changes on the invasive ability of colon cancer cells. MATERIALS AND METHODS:Colon cancer cell lines and human samples derived from a peritoneal metastasis were incubated in a hypercapnic environment, followed by incubation in 5% CO(2). The invasive ability of colon cancer cells incubated with CO(2) were analyzed using an invasion assay system. RESULTS: In comparison with the colon cancer cell lines incubated in 5% CO(2) only, the invasive ability of cells increased in all the colon cancer cell lines subjected to incubation in 20% CO(2) followed by incubation in 5% CO(2), with a concomitant increase in the mRNA expressions of matrix metalloproteinase-2 (MMP2) and MMP9. The invasive capability of peritoneal metastatic cells in the human-derived specimen also increased on CO(2) concentration changes. CONCLUSION:CO(2) concentration changes enhanced the invasive capacity of colon cancer cells.
Authors: Avinoam Nevler; Samantha Z Brown; David Nauheim; Carla Portocarrero; Ulrich Rodeck; Jonathan Bassig; Christopher W Schultz; Grace A McCarthy; Harish Lavu; Theresa P Yeo; Charles J Yeo; Jonathan R Brody Journal: J Am Coll Surg Date: 2020-02-11 Impact factor: 6.113