OBJECTIVE: To observe the inhibition of pulsed electric stimulation on the proliferation of H9c2 cells and the induction on the differentiation of the cells. METHODS: We applied a set of pulse electrical stimulations of different frequencies (0, 1, 5, 10, 50, 100 Hz), different voltage (0, 5, 10, 20, 40, 50 V) and different time (0, 1, 2, 4, 6, 8 h/d) on H9c2 cells in rats. 5-azacytidine was used as an intervention factor control. We detected the proliferation rate of H9c2 cells through MTT assay, the expressions of cardiac troponin T (cTNT) and Oct4 through immunofluorescence cytochemical staining, and the expressions of cTNT and α-MHC mRNA using RT-PCR method. RESULTS: Pulsed electric stimulation inhibited the proliferation of H9c2 cells (P<0.05). The pulsed electric stimulation of 1 Hz, 4 h/d and 10 V was proved optimized in inhibiting the growth of H9c2 cells to the greatest extent but not inducing the death of cells. Two weeks after the optimized pulsed elctric stimulation on H9c2 cells, the expression of Oct4 was reduced (P<0.05), while the expressions of cTNT and α-MHC were still strong. CONCLUSION: Pulsed electric stimulation can not only inhibit the reproduction of myoblast cell line H9c2 in heart tissue of rat embryo, but also induce the differentiation of H9c2 cells. During it, the expressions of cTNT and α-MHC are enhanced but Oct4 is weakened.
OBJECTIVE: To observe the inhibition of pulsed electric stimulation on the proliferation of H9c2 cells and the induction on the differentiation of the cells. METHODS: We applied a set of pulse electrical stimulations of different frequencies (0, 1, 5, 10, 50, 100 Hz), different voltage (0, 5, 10, 20, 40, 50 V) and different time (0, 1, 2, 4, 6, 8 h/d) on H9c2 cells in rats. 5-azacytidine was used as an intervention factor control. We detected the proliferation rate of H9c2 cells through MTT assay, the expressions of cardiac troponin T (cTNT) and Oct4 through immunofluorescence cytochemical staining, and the expressions of cTNT and α-MHC mRNA using RT-PCR method. RESULTS: Pulsed electric stimulation inhibited the proliferation of H9c2 cells (P<0.05). The pulsed electric stimulation of 1 Hz, 4 h/d and 10 V was proved optimized in inhibiting the growth of H9c2 cells to the greatest extent but not inducing the death of cells. Two weeks after the optimized pulsed elctric stimulation on H9c2 cells, the expression of Oct4 was reduced (P<0.05), while the expressions of cTNT and α-MHC were still strong. CONCLUSION: Pulsed electric stimulation can not only inhibit the reproduction of myoblast cell line H9c2 in heart tissue of rat embryo, but also induce the differentiation of H9c2 cells. During it, the expressions of cTNT and α-MHC are enhanced but Oct4 is weakened.