PURPOSE: Human papillomavirus (HPV) is of etiologic significance in the development of oral squamous carcinoma and is noted to result in p16 overexpression. Identification of HPV is clinically important because the presence of HPV has prognostic and epidemiologic associations. Detection of HPV by polymerase chain reaction (PCR) is expensive and not widely accessible. The authors examined p16 immunohistochemistry (IHC) as a surrogate marker for high-risk HPV and its use as an alternative test to PCR. PATIENTS AND METHODS: A retrospective cohort of patients with oral squamous cell carcinoma underwent surgery and then analysis with p16 IHC and HPV PCR. The p16 IHC staining intensity was graded from 0 to 3+, and these results were compared with PCR. Descriptive and frequency statistics were performed by comparing HPV PCR results with p16 IHC, patient age, gender, and outcome. RESULTS: Eighty-one cases were included in the study. Forty-four study patients were men and 37 were women (mean age, 63.9 yr). Forty-five cases (55.6%) had 0 staining, 22 cases (27.2%) had 1+ staining, and 7 cases (8.6%) had 2+ staining. Seven cases (8.6%) had 3+ staining, all of which were positive for HPV serotype 16 by PCR. Three of 7 HPV PCR-positive cases had keratinization typical of an oral cavity location and not the basaloid growth of HPV oropharyngeal tumors. There was a statistical correlation (P < .001) among HPV PCR positivity, 3+ staining, and younger age. CONCLUSION: p16 3+ staining correlates with HPV PCR positivity. p16 IHC is a technically simple and widely available test, and this study establishes the use of p16 IHC as an alternative test to HPV PCR. Given the clinical significance of HPV in oral squamous carcinoma, p16 IHC should be performed in all cases and included in the pathology report.
PURPOSE:Human papillomavirus (HPV) is of etiologic significance in the development of oral squamous carcinoma and is noted to result in p16 overexpression. Identification of HPV is clinically important because the presence of HPV has prognostic and epidemiologic associations. Detection of HPV by polymerase chain reaction (PCR) is expensive and not widely accessible. The authors examined p16 immunohistochemistry (IHC) as a surrogate marker for high-risk HPV and its use as an alternative test to PCR. PATIENTS AND METHODS: A retrospective cohort of patients with oral squamous cell carcinoma underwent surgery and then analysis with p16 IHC and HPV PCR. The p16 IHC staining intensity was graded from 0 to 3+, and these results were compared with PCR. Descriptive and frequency statistics were performed by comparing HPV PCR results with p16 IHC, patient age, gender, and outcome. RESULTS: Eighty-one cases were included in the study. Forty-four study patients were men and 37 were women (mean age, 63.9 yr). Forty-five cases (55.6%) had 0 staining, 22 cases (27.2%) had 1+ staining, and 7 cases (8.6%) had 2+ staining. Seven cases (8.6%) had 3+ staining, all of which were positive for HPV serotype 16 by PCR. Three of 7 HPV PCR-positive cases had keratinization typical of an oral cavity location and not the basaloid growth of HPV oropharyngeal tumors. There was a statistical correlation (P < .001) among HPV PCR positivity, 3+ staining, and younger age. CONCLUSION:p16 3+ staining correlates with HPV PCR positivity. p16 IHC is a technically simple and widely available test, and this study establishes the use of p16 IHC as an alternative test to HPV PCR. Given the clinical significance of HPV in oral squamous carcinoma, p16 IHC should be performed in all cases and included in the pathology report.
Authors: Tatyana Isayeva; Jie Xu; Camille Ragin; Qian Dai; Tiffiny Cooper; William Carroll; Dan Dayan; Marilena Vered; Bruce Wenig; Eben Rosenthal; William Grizzle; Joshua Anderson; Christopher D Willey; Eddy S Yang; Margaret Brandwein-Gensler Journal: Mod Pathol Date: 2014-12-19 Impact factor: 7.842
Authors: Elizabeth A Simonik; Ying Cai; Katherine N Kimmelshue; Dana M Brantley-Sieders; Holli A Loomans; Claudia D Andl; Grant M Westlake; Victoria M Youngblood; Jin Chen; Wendell G Yarbrough; Brandee T Brown; Lalitha Nagarajan; Stephen J Brandt Journal: PLoS One Date: 2016-10-25 Impact factor: 3.240
Authors: Anna Schuh; Helene Dreau; Samantha J L Knight; Kate Ridout; Tuba Mizani; Dimitris Vavoulis; Richard Colling; Pavlos Antoniou; Erika M Kvikstad; Melissa M Pentony; Angela Hamblin; Andrew Protheroe; Marina Parton; Ketan A Shah; Zsolt Orosz; Nick Athanasou; Bass Hassan; Adrienne M Flanagan; Ahmed Ahmed; Stuart Winter; Adrian Harris; Ian Tomlinson; Niko Popitsch; David Church; Jenny C Taylor Journal: Cold Spring Harb Mol Case Stud Date: 2018-04-02
Authors: M Pogorzelski; S Ting; T C Gauler; F Breitenbuecher; I Vossebein; S Hoffarth; J Markowetz; S Lang; C Bergmann; S Brandau; J A Jawad; K W Schmid; M Schuler; S Kasper Journal: Cell Death Dis Date: 2014-02-27 Impact factor: 8.469