BACKGROUND: Recent research suggest that B and plasma cells may play an important role in the pathogenesis of chronic rhinosinusitis with nasal polyposis (CRSwNP). The purpose of this study was to subcharacterize the B cell response in the sinus mucosa of control and CRS patients. METHODS: Representative tissue samples and peripheral blood samples were obtained from controls, CRS without nasal polyps (CRSsNP) and CRSwNP. Using single-cell suspension flow cytometry these samples were analyzed for overall and stage-specific B and plasma cell percentages. RESULTS: Both atopic and nonatopic CRSwNP patients showed an increase in local numbers of naive, active, and memory B cells compared to controls. CRSsNP patients only showed local elevations of naive B cells. Plasma cells were only significantly elevated in the sinus tissue of atopic CRSwNP patients. These local tissue increases did not correlate with increased numbers of circulating B cells. CONCLUSION: This study provides further evidence of an important role of B cells in CRSwNP patients. The local increase appears to be independent of a systemic response.
BACKGROUND: Recent research suggest that B and plasma cells may play an important role in the pathogenesis of chronic rhinosinusitis with nasal polyposis (CRSwNP). The purpose of this study was to subcharacterize the B cell response in the sinus mucosa of control and CRSpatients. METHODS: Representative tissue samples and peripheral blood samples were obtained from controls, CRS without nasal polyps (CRSsNP) and CRSwNP. Using single-cell suspension flow cytometry these samples were analyzed for overall and stage-specific B and plasma cell percentages. RESULTS: Both atopic and nonatopic CRSwNP patients showed an increase in local numbers of naive, active, and memory B cells compared to controls. CRSsNP patients only showed local elevations of naive B cells. Plasma cells were only significantly elevated in the sinus tissue of atopic CRSwNP patients. These local tissue increases did not correlate with increased numbers of circulating B cells. CONCLUSION: This study provides further evidence of an important role of B cells in CRSwNP patients. The local increase appears to be independent of a systemic response.
Authors: William W Carroll; Rodney J Schlosser; Brendan P O'Connell; Zachary M Soler; Jennifer K Mulligan Journal: Int Forum Allergy Rhinol Date: 2016-01-11 Impact factor: 3.858
Authors: Sarah E Smith; Rodney J Schlosser; James R Yawn; Jose L Mattos; Zachary M Soler; Jennifer K Mulligan Journal: Am J Rhinol Allergy Date: 2017-11-01 Impact factor: 2.467
Authors: Jennifer K Mulligan; Brendan P O'Connell; Whitney Pasquini; Ryan M Mulligan; Sarah Smith; Zachary M Soler; Carl Atkinson; Rodney J Schlosser Journal: Int Forum Allergy Rhinol Date: 2017-06-02 Impact factor: 3.858
Authors: Robert J Taylor; Rodney J Schlosser; Zachary M Soler; Jose L Mattos; Jennifer K Mulligan Journal: Int Forum Allergy Rhinol Date: 2018-05-02 Impact factor: 3.858
Authors: Jennifer K Mulligan; Whitney N Pasquini; William W Carroll; Tucker Williamson; Nicholas Reaves; Kunal J Patel; Elliott Mappus; Rodney J Schlosser; Carl Atkinson Journal: PLoS One Date: 2017-10-18 Impact factor: 3.240