Literature DB >> 23637482

Molecular Characterization of Classical swine fever virus Involved in the Outbreak in Mizoram.

N N Barman1, R S Gupt, D P Bora, R S Kataria, A K Tiwari, P Roychoudhury.   

Abstract

Classical swine fever is the most insidious and devastating disease of pigs and wild boars. The virus is closely related to the other members of the genus Pestivirus. The outbreak recorded in Mizoram, India was strategically important as the state shares porous international boundary with East Asian countries. Both immunodiagnostic and molecular techniques were used to confirm the involvement of Classical swine fever virus (CSFV) in this outbreak. Sandwich ELISA and direct FAT could detect CSFV in the tissue samples. RT-nPCR specifically amplified E2 and 5'NTR product of 271 bp. Phylogenetic analysis showed, that the Mizoram isolate (MZ4/69) was very close to the Chinese strain Shimen-HVRI (93.0%) rather than other Indian isolate (CSF-30-03). Present study provides a valuable sequence based molecular data on Indian isolate of CSFV and will be useful in investigation on transmission of such disease from neighbour countries.

Entities:  

Keywords:  Classical swine fever virus ELISA; RT-PCR; Sequence analysis

Year:  2010        PMID： 23637482      PMCID： PMC3550764          DOI： 10.1007/s13337-010-0006-z

Source DB:  PubMed          Journal:  Indian J Virol        ISSN： 0970-2822


  10 in total

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Journal:  J Virol Methods       Date:  1998-11       Impact factor: 2.014

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Journal:  J Gen Virol       Date:  1996-06       Impact factor: 3.891

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Journal:  Vet Rec       Date:  1985-03-16       Impact factor: 2.695
  10 in total
  6 in total

1.  Changing pattern of classical swine fever virus genogroup from classical 1.1 to emerging 2.2 in India.

Authors:  Vinod Kumar Singh; Kaushal Kishor Rajak; Ravi Kumar; Sachin D Raut; Arpit Saxena; Dhanavellu Muthuchelvan; Raj Kumar Singh; Awadh Bihari Pandey
Journal:  Virusdisease       Date:  2017-03-20

2.  Molecular characterization of E2 glycoprotein of classical swine fever virus: adaptation and propagation in porcine kidney cells.

Authors:  Rakesh Kumar; Nagendra N Barman; Elina Khatoon; Gitika Rajbongshi; Nipu Deka; Sudhir Morla; Sachin Kumar
Journal:  In Vitro Cell Dev Biol Anim       Date:  2015-01-01       Impact factor: 2.416

Review 3.  Classical swine fever in India: current status and future perspective.

Authors:  Vinod Kumar Singh; Kaushal Kishore Rajak; Amit Kumar; Sharad Kumar Yadav
Journal:  Trop Anim Health Prod       Date:  2018-05-04       Impact factor: 1.559

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Authors:  A K Chakraborty; A Karam; P Mukherjee; L Barkalita; P Borah; S Das; R Sanjukta; K Puro; S Ghatak; I Shakuntala; I Sharma; R G Laha; A Sen
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Authors:  Anuj Ahuja; Uttaran Bhattacharjee; Amit Kumar Chakraborty; Amarjit Karam; Sandeep Ghatak; Kekungu Puro; Samir Das; Ingudam Shakuntala; Nidhi Srivastava; S V Ngachan; Arnab Sen
Journal:  Genome Announc       Date:  2015-01-22

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Authors:  David Malswamkima; T K Rajkhowa; Rajesh Chandra; T K Dutta
Journal:  Vet World       Date:  2015-01-24
  6 in total

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