Literature DB >> 2363726

Expression of a human insulin-like growth factor II cDNA in NIH-3T3 cells.

D M Buergisser1, B V Roth, C Luethi, H P Gerber, A Honegger, R E Humbel.   

Abstract

Recombinant human insulin-like growth factor II (IGF-II) was produced in NIH-3T3 cells transfected with a plasmid containing a construct encoding the signal peptide and the sequence of mature human IGF II. Successfully transfected clones secreted correctly processed recombinant human IGF II at rates of about 100ng per 24 hours per 10(6) cells. The biological activity of the purified recombinant human IGF II exhibited similar potencies as standard human IGF II isolated from serum in radio-receptorassays as well as in thymidine incorporation assays.

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Year:  1990        PMID： 2363726     DOI： 10.1016/0006-291x(90)91968-x

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  1 in total

1.  Accurate processing and secretion in the baculovirus expression system of an erythroid-cell-stimulating factor consisting of a chimaera of insulin-like growth factor II and an insect insulin-like peptide.

Authors:  L F Congote; Q Li
Journal:  Biochem J       Date:  1994-04-01       Impact factor: 3.857
  1 in total

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