BACKGROUND: Diabetes can lead to impaired wound healing and skin grafts used surgically for diabetic wounds are often complicated with necrosis, although different therapies have been proposed. Adipose-derived stem cells (ASCs) participate in tissue repair processes and may have a role during impaired wound healing. In this study, autologous transplantation of ASCs was used to determine if it increases angiogenesis and skin graft survival and enhances wound healing in diabetic rats. METHODS: Adipose-derived stem cells were successfully isolated and cultured. A full-thickness skin graft model was used to determine the effects of locally administered ASCs in 10 rats rendered diabetic (group 1), whereas 10 others served as controls (group 2). Histological examination of skin grafts followed after 1 week. Additionally, immunohistochemical staining intensity of vascular endothelial growth factor (VEGF) and transforming growth factor β3 (TGF-β3) was assessed in all grafts. RESULTS: The gross and histological results showed significantly increased survival, angiogenesis, and epithelialization. Mean area of graft necrosis was significantly less in group 1 than in group 2 (7.49% vs 39.67%, P < 0.001). Statistically significant increase of capillary density, collagen intensity, VEGF, and TGF-β3 expression was noted in group 1 compared with group 2. CONCLUSIONS: These findings suggest that autologous ASC transplantation can enhance skin graft survival in diabetic rats through differentiation, vasculogenesis, and secretion of growth factors such as VEGF and TGF-β3. This might represent a novel therapeutic approach in skin graft surgery for diabetic wounds.
BACKGROUND:Diabetes can lead to impaired wound healing and skin grafts used surgically for diabetic wounds are often complicated with necrosis, although different therapies have been proposed. Adipose-derived stem cells (ASCs) participate in tissue repair processes and may have a role during impaired wound healing. In this study, autologous transplantation of ASCs was used to determine if it increases angiogenesis and skin graft survival and enhances wound healing in diabeticrats. METHODS: Adipose-derived stem cells were successfully isolated and cultured. A full-thickness skin graft model was used to determine the effects of locally administered ASCs in 10 rats rendered diabetic (group 1), whereas 10 others served as controls (group 2). Histological examination of skin grafts followed after 1 week. Additionally, immunohistochemical staining intensity of vascular endothelial growth factor (VEGF) and transforming growth factor β3 (TGF-β3) was assessed in all grafts. RESULTS: The gross and histological results showed significantly increased survival, angiogenesis, and epithelialization. Mean area of graft necrosis was significantly less in group 1 than in group 2 (7.49% vs 39.67%, P < 0.001). Statistically significant increase of capillary density, collagen intensity, VEGF, and TGF-β3 expression was noted in group 1 compared with group 2. CONCLUSIONS: These findings suggest that autologous ASC transplantation can enhance skin graft survival in diabeticrats through differentiation, vasculogenesis, and secretion of growth factors such as VEGF and TGF-β3. This might represent a novel therapeutic approach in skin graft surgery for diabetic wounds.
Authors: Stephen M Cronk; Molly R Kelly-Goss; H Clifton Ray; Thomas A Mendel; Kyle L Hoehn; Anthony C Bruce; Bijan K Dey; Alexander M Guendel; Daniel N Tavakol; Ira M Herman; Shayn M Peirce; Paul A Yates Journal: Stem Cells Transl Med Date: 2015-03-13 Impact factor: 6.940
Authors: Gangaraju Rajashekhar; Ahmed Ramadan; Chandrika Abburi; Breedge Callaghan; Dmitry O Traktuev; Carmella Evans-Molina; Raj Maturi; Alon Harris; Timothy S Kern; Keith L March Journal: PLoS One Date: 2014-01-09 Impact factor: 3.240
Authors: Stefan Hacker; Rainer Mittermayr; Stefanie Nickl; Thomas Haider; Diana Lebherz-Eichinger; Lucian Beer; Andreas Mitterbauer; Harald Leiss; Matthias Zimmermann; Thomas Schweiger; Claudia Keibl; Helmut Hofbauer; Christian Gabriel; Mariann Pavone-Gyöngyösi; Heinz Redl; Erwin Tschachler; Michael Mildner; Hendrik Jan Ankersmit Journal: Sci Rep Date: 2016-04-29 Impact factor: 4.379