BACKGROUND/AIMS: β-actin is known to be involved in motility of cancer cells. However, the association between β-actin expression and metastasis of gastric cancer remains unclear. METHODOLOGY: We enrolled 40 gastric cancer patients who underwent gastrectomy and evaluated the expression of β-actin in gastric cancer tissues and the corresponding normal mucosa by the analysis of Western blotting. To further study the function of β-actin in cancer cell migration, wound healing assay was performed in SGC7901 transfected with β-actin siRNA. RESULTS: The level of β-actin was significantly higher in gastric cancer tissues than in the corresponding normal mucosa. The correlation between β-actin expression and the local lymph node metastasis was statistically significant, which demonstrated that overexpression of β-actin might contribute to the local lymph node metastasis of gastric cancer. Interference of β-actin expression could inhibit migration of SGC7901 cells. CONCLUSIONS: β-actin, the most conservative and dynamic protein, is overexpressed in gastric cancer tissues and may play a crucial role in metastasis of gastric cancer.
BACKGROUND/AIMS: β-actin is known to be involved in motility of cancer cells. However, the association between β-actin expression and metastasis of gastric cancer remains unclear. METHODOLOGY: We enrolled 40 gastric cancerpatients who underwent gastrectomy and evaluated the expression of β-actin in gastric cancer tissues and the corresponding normal mucosa by the analysis of Western blotting. To further study the function of β-actin in cancer cell migration, wound healing assay was performed in SGC7901 transfected with β-actin siRNA. RESULTS: The level of β-actin was significantly higher in gastric cancer tissues than in the corresponding normal mucosa. The correlation between β-actin expression and the local lymph node metastasis was statistically significant, which demonstrated that overexpression of β-actin might contribute to the local lymph node metastasis of gastric cancer. Interference of β-actin expression could inhibit migration of SGC7901 cells. CONCLUSIONS: β-actin, the most conservative and dynamic protein, is overexpressed in gastric cancer tissues and may play a crucial role in metastasis of gastric cancer.
Authors: Amber Gonda; Nanxia Zhao; Jay V Shah; Jake N Siebert; Srujanesh Gunda; Berk Inan; Mijung Kwon; Steven K Libutti; Prabhas V Moghe; Nicola L Francis; Vidya Ganapathy Journal: Front Oncol Date: 2021-11-18 Impact factor: 6.244
Authors: Zaynab Al-Eisawi; Philip Beale; Charles Chan; Jun Qing Yu; Nicholas Proschogo; Mark Molloy; Fazlul Huq Journal: BMC Cancer Date: 2016-08-26 Impact factor: 4.430