Literature DB >> 2363494

A sensitive radioisotopic method for the measurement of NAD(P)H: its application to the assay of metabolites and enzymatic activities.

A Sener1, W J Malaisse.   

Abstract

A radioisotopic method for the assay of NADH or NADPH is presented, which is based on the conversion of 2-[U-14C]ketoglutarate to 14C-labeled glutamate in the reaction catalyzed by glutamate dehydrogenase. The efficiency of the method is close to 75%, its precision (coefficient of variation) close to 5%, and its sensitivity close to 0.1 pmol/sample. This simple and rapid method can be applied to the measurement of several metabolites and enzymatic activities. In the present study, its application to the assay of sorbitol, 3-hydroxybutyrate, glutamate dehydrogenase, 3-hydroxybutyrate dehydrogenase, and glyceraldehyde-3-phosphate dehydrogenase is documented.

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Year:  1990        PMID： 2363494     DOI： 10.1016/0003-2697(90)90073-i

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  10 in total

1.  Impaired FAD-glycerophosphate dehydrogenase activity in islet and liver homogenates of fa/fa rats.

Authors:  J Rasschaert; F Malaisse-Lagae; A Sener; V Leclercq-Meyer; L Herberg; W J Malaisse
Journal:  Mol Cell Biochem       Date:  1994-06-29       Impact factor: 3.396

2.  Effect of streptozotocin and nicotinamide upon FAD-glycerophosphate dehydrogenase activity and insulin release in purified pancreatic B-cells.

Authors:  J Rasschaert; Z Ling; W J Malaisse
Journal:  Mol Cell Biochem       Date:  1993-03-24       Impact factor: 3.396

3.  Deficient activity of FAD-linked glycerophosphate dehydrogenase in islets of GK rats.

Authors:  C G Ostenson; S M Abdel-Halim; J Rasschaert; F Malaisse-Lagae; S Meuris; A Sener; S Efendic; W J Malaisse
Journal:  Diabetologia       Date:  1993-08       Impact factor: 10.122

4.  Streptozotocin-induced FAD-glycerophosphate dehydrogenase suppression in pancreatic islets. Relationship with the severity and duration of hyperglycaemia and resistance to insulin or riboflavin treatment.

Authors:  J Rasschaert; W J Malaisse
Journal:  Acta Diabetol       Date:  1993       Impact factor: 4.280

5.  Enzymatic, metabolic and secretory patterns in human islets of type 2 (non-insulin-dependent) diabetic patients.

Authors:  J Fernandez-Alvarez; I Conget; J Rasschaert; A Sener; R Gomis; W J Malaisse
Journal:  Diabetologia       Date:  1994-02       Impact factor: 10.122

6.  Interference of glycogenolysis with glycolysis in pancreatic islets from glucose-infused rats.

Authors:  W J Malaisse; C Maggetto; V Leclercq-Meyer; A Sener
Journal:  J Clin Invest       Date:  1993-02       Impact factor: 14.808

7.  Dissociated effects of 2-deoxy-D-glucose on D-[2-3H]glucose and D-[5-3H]glucose conversion into 3HOH in rat erythrocytes.

Authors:  B Manuel y Keenoy; D Zähner; W J Malaisse
Journal:  Biochem J       Date:  1992-12-01       Impact factor: 3.857

8.  Hexose metabolism in pancreatic islets. Glucose-induced and Ca(2+)-dependent activation of FAD-glycerophosphate dehydrogenase.

Authors:  J Rasschaert; W J Malaisse
Journal:  Biochem J       Date:  1991-09-01       Impact factor: 3.857

9.  Evidence that adrenal hexose-6-phosphate dehydrogenase can effect microsomal P450 cytochrome steroidogenic enzymes.

Authors:  Christy A Foster; Gail J Mick; Xudong Wang; Kenneth McCormick
Journal:  Biochim Biophys Acta       Date:  2013-05-09

10.  The relative roles of advanced glycation, oxidation and aldose reductase inhibition in the development of experimental diabetic nephropathy in the Sprague-Dawley rat.

Authors:  T Soulis-Liparota; M E Cooper; M Dunlop; G Jerums
Journal:  Diabetologia       Date:  1995-04       Impact factor: 10.122
  10 in total

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