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Literature DB >> 236278

Thiolases of Escherichia coli: purification and chain length specificities.

Abstract

The presence of only one thiolase (EC 2.3.1.9) in wild-type Escherichia coli induced for enzymes of beta oxidation was demonstrated. A different thiolase was shown to be present in a mutant constitutive for the enzymes of butyrate degradation. The two thiolases were purified to near homogeneity by a simple two-step procedure and were found to be associated with different proteins as shown by gel electrophoresis. The thiolase isolated from induced wild-type Escherichia coli cell was active on beta-ketoacyl-coenzyme A derivatives containing 4 to 16 carbons, but exhibited optimal activity with medium-chain substrates. In contrast, the thiolase isolated from the constitutive mutant was shown to be specific for acetoacetyl-coenzyme A.

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Year: 1975 PMID： 236278 PMCID： PMC246071 DOI： 10.1128/jb.122.2.407-411.1975

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

14 in total

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Authors: G Pauli; P Overath
Journal: Eur J Biochem Date: 1972-09-25

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Authors: B Middleton
Journal: Biochem J Date: 1973-04 Impact factor: 3.857

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Authors: H Schulz
Journal: J Biol Chem Date: 1974-05-10 Impact factor: 5.157

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Authors: Y Mazzei; R Negrel; G Ailhaud
Journal: Biochim Biophys Acta Date: 1970-10-14

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Authors: B Middleton
Journal: Biochem Biophys Res Commun Date: 1972-01-31 Impact factor: 3.575

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Authors: W Seubert; I Lamberts; R Kramer; B Ohly
Journal: Biochim Biophys Acta Date: 1968-12-18

15 in total

1. Engineered reversal of the β-oxidation cycle for the synthesis of fuels and chemicals.

Authors: Clementina Dellomonaco; James M Clomburg; Elliot N Miller; Ramon Gonzalez
Journal: Nature Date: 2011-08-10 Impact factor: 49.962

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Authors: A Pramanik; S Pawar; E Antonian; H Schulz
Journal: J Bacteriol Date: 1979-01 Impact factor: 3.490

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Authors: W D Nunn
Journal: Microbiol Rev Date: 1986-06

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Authors: Anastasia A Pantazaki; Andrea K Ioannou; Dimitrios A Kyriakidis
Journal: Mol Cell Biochem Date: 2005-01 Impact factor: 3.396

5. Genetic evaluation of physiological functions of thiolase isoenzymes in the n-alkalane-assimilating yeast Candida tropicalis.

Authors: N Kanayama; M Ueda; H Atomi; A Tanaka
Journal: J Bacteriol Date: 1998-02 Impact factor: 3.490

6. Genetically modified strains of Ralstonia eutropha H16 with β-ketothiolase gene deletions for production of copolyesters with defined 3-hydroxyvaleric acid contents.

Authors: Nicole Lindenkamp; Elena Volodina; Alexander Steinbüchel
Journal: Appl Environ Microbiol Date: 2012-05-25 Impact factor: 4.792

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Authors: J F Binstock; A Pramanik; H Schulz
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Journal: Proc Natl Acad Sci U S A Date: 1982-08 Impact factor: 11.205

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Journal: Appl Environ Microbiol Date: 1995-07 Impact factor: 4.792

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Authors: W J O'Brien; F E Frerman
Journal: J Bacteriol Date: 1977-11 Impact factor: 3.490