Janibacter cremeus sp. nov., an actinobacterium isolated from sea sediment.

A novel Gram-stain-positive actinobacterium, designated HR08-44(T), was isolated from a sea-sediment sample collected from the foreshore of Rishiri Island, Japan, and its taxonomic position was investigated by a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain HR08-44(T) was closely related to the members of the genus Janibacter, with pairwise sequence similarities of 97.3-98.8 %. Strain HR08-44(T) had peptidoglycan type A1γ, with meso-diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinone was MK-8(H4) and the major fatty acids were iso-C16 : 0, C17 : 1ω8c, C17 : 0 and C18 : 1ω9c. These data supported the affiliation of the novel strain to the genus Janibacter. Meanwhile, the results of DNA-DNA hybridization and physiological and biochemical tests indicated that strain HR08-44(T) can be distinguished from recognized species of the genus Janibacter. Therefore, strain HR08-44(T) represents a novel species of the genus Janibacter, for which the name Janibacter cremeus sp. nov. is proposed; the type strain is HR08-44(T) ( = NBRC 107693(T) = DSM 26154(T)).