| Literature DB >> 23625059 |
Ajay K Singh1, Amita Sachdeva, Jeffrey A Degrasse, Timothy R Croley, Larry H Stanker, David Hodge, Shashi K Sharma.
Abstract
Botulinum neurotoxins are produced as a toxin complex (TC) which consists of neurotoxin (NT) and neurotoxin associated proteins. The characterization of NT in its native state is an essential step for developing diagnostics and therapeutic countermeasures against botulism. The presence of NT genes was validated by PCR amplification of toxin specific fragments from genomic DNA of Clostridium botulinum strain PS-5 which indicated the presence of both serotype A and B genes on PS-5 genome. Further, TC was purified and characterized by Western blotting, Digoxin-enzyme linked immunosorbent assay, endopeptidase activity assay, and Liquid chromatography-Mass spectrometry. The data showed the presence of serotype A specific neurotoxin. Based on the analysis of neurotoxin genes and characterization of TC, PS-5 strain appears as a serotype A (B) strain of C. botulinum which produces only serotype A specific TC in the cell culture medium.Entities:
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Year: 2013 PMID： 23625059 DOI： 10.1007/s10930-013-9486-1
Source DB: PubMed Journal: Protein J ISSN： 1572-3887 Impact factor: 2.371