PURPOSE: The aim of this study was to determine the expression and function of miR-509-5p in renal cell carcinoma (RCC). MATERIALS AND METHODS: In this research, we have conducted quantitative real-time polymerase chain reaction (qRT-PCR) assay to determine the expression level of miR-509-5p in tissues and plasma from renal cell carcinoma patients. We preformed in vitro migration scratch assay, flow cytometry analysis and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay to determine the exact function of miR-509-5p. RESULTS: We evaluated the expression level of miR-509-5p in RCC tissues and paired adjacent normal tissues from 42 patients and found that miR-509-5p expression in 42 RCC specimens was significantly down-regulated compared to that in adjacent normal tissue. Furthermore, the level of miR-509-5p in RCC patients' plasma was significantly lower than that in control plasma. In addition, the overexpression of miR-509-5p suppressed the proliferation of RCC cell (786-0), induced cell apoptosis and inhibited cell migration in vitro. CONCLUSION: In this study, we have shown that miR-509-5p played an important role in RCC by inhibiting cell proliferation and migration and by promoting cell apoptosis. In addition, miR-509-5p expression was significantly lower in RCC patient plasma compared to that in normal individuals.
PURPOSE: The aim of this study was to determine the expression and function of miR-509-5p in renal cell carcinoma (RCC). MATERIALS AND METHODS: In this research, we have conducted quantitative real-time polymerase chain reaction (qRT-PCR) assay to determine the expression level of miR-509-5p in tissues and plasma from renal cell carcinomapatients. We preformed in vitro migration scratch assay, flow cytometry analysis and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay to determine the exact function of miR-509-5p. RESULTS: We evaluated the expression level of miR-509-5p in RCC tissues and paired adjacent normal tissues from 42 patients and found that miR-509-5p expression in 42 RCC specimens was significantly down-regulated compared to that in adjacent normal tissue. Furthermore, the level of miR-509-5p in RCCpatients' plasma was significantly lower than that in control plasma. In addition, the overexpression of miR-509-5p suppressed the proliferation of RCC cell (786-0), induced cell apoptosis and inhibited cell migration in vitro. CONCLUSION: In this study, we have shown that miR-509-5p played an important role in RCC by inhibiting cell proliferation and migration and by promoting cell apoptosis. In addition, miR-509-5p expression was significantly lower in RCCpatient plasma compared to that in normal individuals.
Authors: Nicole M White; Anna Bui; Salvador Mejia-Guerrero; Julie Chao; Antoninus Soosaipillai; Youssef Youssef; Marina Mankaruos; R John Honey; Robert Stewart; Kenneth T Pace; Linda Sugar; Eleftherios P Diamandis; Jules Doré; George M Yousef Journal: Biol Chem Date: 2010-04 Impact factor: 3.915
Authors: Yinghong Pan; Gordon Robertson; Lykke Pedersen; Emilia Lim; Anadulce Hernandez-Herrera; Amy C Rowat; Sagar L Patil; Clara K Chan; Yunfei Wen; Xinna Zhang; Upal Basu-Roy; Alka Mansukhani; Andy Chu; Payal Sipahimalani; Reanne Bowlby; Denise Brooks; Nina Thiessen; Cristian Coarfa; Yussanne Ma; Richard A Moore; Jacquie E Schein; Andrew J Mungall; Jinsong Liu; Chad V Pecot; Anil K Sood; Steven J M Jones; Marco A Marra; Preethi H Gunaratne Journal: Oncotarget Date: 2016-05-03