Literature DB >> 2361949

In vitro characterization of two different Phycomyces blakesleeanus mutants with impaired phytoene desaturation.

A Schmidt1, G Sandmann.   

Abstract

In vitro phytoene desaturation was investigated in two Phycomyces blakesleeanus mutants, C5 and S442, in which phytoene is accumulated instead of beta-carotene. For strain C5 but not strain S442 the phenotypic block of phytoene conversion could be overcome in vitro by the addition of Tween 40. Immunodetection of phytoene desaturase revealed in all cases the presence of a 40-kilodalton protein.

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Year:  1990        PMID: 2361949      PMCID: PMC213398          DOI: 10.1128/jb.172.7.4103-4105.1990

Source DB:  PubMed          Journal:  J Bacteriol        ISSN: 0021-9193            Impact factor:   3.490


  8 in total

1.  THE PHYTOENE CONTENT OF TOMATOES.

Authors:  G Mackinney; C M Rick; J A Jenkins
Journal:  Proc Natl Acad Sci U S A       Date:  1956-07       Impact factor: 11.205

Review 2.  Chromosomal loci of Neurospora crassa.

Authors:  D D Perkins; A Radford; D Newmeyer; M Björkman
Journal:  Microbiol Rev       Date:  1982-12

3.  Immunological detection of phytoene desaturase in algae and higher plants using an antiserum raised against a bacterial fusion-gene construct.

Authors:  A Schmidt; G Sandmann; G A Armstrong; J E Hearst; P Böger
Journal:  Eur J Biochem       Date:  1989-09-15

4.  The gene crtI mediates the conversion of phytoene into colored carotenoids in Rhodopseudomonas capsulata.

Authors:  G Giuliano; D Pollock; P A Scolnik
Journal:  J Biol Chem       Date:  1986-10-05       Impact factor: 5.157

5.  In vitro carotenogenesis and characterization of the phytoene desaturase reaction in Anacystis.

Authors:  G Sandmann; S Kowalczyk
Journal:  Biochem Biophys Res Commun       Date:  1989-09-15       Impact factor: 3.575

6.  Carotenoid biosynthesis in photosynthetic bacteria. Genetic characterization of the Rhodobacter capsulatus CrtI protein.

Authors:  G E Bartley; P A Scolnik
Journal:  J Biol Chem       Date:  1989-08-05       Impact factor: 5.157

7.  Carotenes and retinal in Phycomyces mutants.

Authors:  G Meissner; M Delbruck
Journal:  Plant Physiol       Date:  1968-08       Impact factor: 8.340

8.  Nucleotide sequence, organization, and nature of the protein products of the carotenoid biosynthesis gene cluster of Rhodobacter capsulatus.

Authors:  G A Armstrong; M Alberti; F Leach; J E Hearst
Journal:  Mol Gen Genet       Date:  1989-04
  8 in total
  2 in total

1.  Genomic perspective on the photobiology of Halobacterium species NRC-1, a phototrophic, phototactic, and UV-tolerant haloarchaeon.

Authors:  S Dassarma; S P Kennedy; B Berquist; W Victor Ng; N S Baliga; J L Spudich; M P Krebs; J A Eisen; C H Johnson; L Hood
Journal:  Photosynth Res       Date:  2001       Impact factor: 3.573

2.  Purification and reactivation of recombinant Synechococcus phytoene desaturase from an overexpressing strain of Escherichia coli.

Authors:  P D Fraser; H Linden; G Sandmann
Journal:  Biochem J       Date:  1993-05-01       Impact factor: 3.857

  2 in total

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