AIMS: To examine whether overexpression of peroxisome proliferator activated receptor-gamma coactivator-1 alpha (PGC-1α) can prevent apoptosis in adipose-derived stem cells (ASCs) by reducing reactive oxygen species (ROS) production and enhancing mitochondrial function in a diabetic environment. METHODS: After the isolation, expansion and characterisation of rat ASCs, we overexpressed PGC-1α in ASCs using an adenoviral vector encoding green fluorescent protein (GFP) or PGC-1α and tested the apoptotic effect under conditions of high glucose, hypoxia and serum deprivation. The production of intracellular ROS and mitochondrial ROS was evaluated using dihydroethidium and CM-H2XRos fluorescent probes. RESULTS: Under conditions of high glucose, hypoxia and serum deprivation, the overexpression of PGC-1α in ASCs decreased apoptosis and led to an increased survival rate. The ASCs modified with PGC-1α produced lower intracellular and mitochondrial ROS. The mitochondrial morphology and structure in the PGC-1α-ASC group remained relatively complete compared with the control group. CONCLUSIONS: These results reveal a crucial protective role for PGC-1α in the treatment of diabetes mellitus and its complications using stem cells therapy.
AIMS: To examine whether overexpression of peroxisome proliferator activated receptor-gamma coactivator-1 alpha (PGC-1α) can prevent apoptosis in adipose-derived stem cells (ASCs) by reducing reactive oxygen species (ROS) production and enhancing mitochondrial function in a diabetic environment. METHODS: After the isolation, expansion and characterisation of rat ASCs, we overexpressed PGC-1α in ASCs using an adenoviral vector encoding green fluorescent protein (GFP) or PGC-1α and tested the apoptotic effect under conditions of high glucose, hypoxia and serum deprivation. The production of intracellular ROS and mitochondrial ROS was evaluated using dihydroethidium and CM-H2XRos fluorescent probes. RESULTS: Under conditions of high glucose, hypoxia and serum deprivation, the overexpression of PGC-1α in ASCs decreased apoptosis and led to an increased survival rate. The ASCs modified with PGC-1α produced lower intracellular and mitochondrial ROS. The mitochondrial morphology and structure in the PGC-1α-ASC group remained relatively complete compared with the control group. CONCLUSIONS: These results reveal a crucial protective role for PGC-1α in the treatment of diabetes mellitus and its complications using stem cells therapy.
Authors: Niels A J Cremers; Ditte M S Lundvig; Stephanie C M van Dalen; Rik F Schelbergen; Peter L E M van Lent; Walter A Szarek; Raymond F Regan; Carine E Carels; Frank A D T G Wagener Journal: Int J Mol Sci Date: 2014-10-08 Impact factor: 5.923
Authors: Yan Chen; Yu Ma; Ning Li; Hongyan Wang; Bing Chen; Ziwen Liang; Rui Ren; Debin Lu; Johnson Boey; David G Armstrong; Wuquan Deng Journal: Stem Cell Res Ther Date: 2018-04-10 Impact factor: 6.832