BACKGROUND: Structural and functional oligodendrocyte deficits as well as impaired myelin integrity have been described in affective disorders and schizophrenia, and may disturb the connectivity between disease-relevant brain regions. Olig1, an oligodendroglial transcription factor, might be important in this context, but has not been systematically studied so far. METHODS: Nissl- and Olig1-stained oligodendrocytes were quantified in the pregenual anterior cingulate (pACC)/dorsolateral prefrontal cortex (DLPFC), and adjacent white matter of patients with major depressive disorder (MDD, n = 9), bipolar disorder (BD, n = 8), schizophrenia (SZ, n = 13), and matched controls (n = 16). Potential downstream effects of increased Olig1-expression were analyzed. Antidepressant drug effects on Olig1-expression were further explored in OLN-93 oligodendrocyte cultures. RESULTS: Nissl-stainings of both white matter regions showed a 19-27% reduction of total oligodendrocyte densities in MDD and BD, but not in SZ. In contrast, nuclear Olig1-immunoreactivity was elevated in MDD in the pACC-adjacent white matter (left: p = 0.008; right: p = 0.018); this effect tended to increase with antidepressant dosage (r = 0.631, p = 0.069). This reactive increase of Olig1 was confirmed by partly dose-dependent effects of imipramine and amitriptyline in oligodendrocyte cultures. Correspondingly, MBP expression in the pACC-adjacent white matter tended to increase with antidepressant dosage (r = 0.637, p = 0.065). Other tested brain regions showed no diagnosis-dependent differences regarding Olig1-immunoreactivity. CONCLUSIONS: Since nuclear Olig1-expression marks oligodendrocyte precursor cells, its increased expression along with reduced total oligodendrocyte densities (Nissl-stained) in the pACC-adjacent white matter of MDD patients might indicate a (putatively medication-boosted) regenerative attempt to compensate oligodendrocyte loss.
BACKGROUND:Structural and functional oligodendrocyte deficits as well as impaired myelin integrity have been described in affective disorders and schizophrenia, and may disturb the connectivity between disease-relevant brain regions. Olig1, an oligodendroglial transcription factor, might be important in this context, but has not been systematically studied so far. METHODS: Nissl- and Olig1-stained oligodendrocytes were quantified in the pregenual anterior cingulate (pACC)/dorsolateral prefrontal cortex (DLPFC), and adjacent white matter of patients with major depressive disorder (MDD, n = 9), bipolar disorder (BD, n = 8), schizophrenia (SZ, n = 13), and matched controls (n = 16). Potential downstream effects of increased Olig1-expression were analyzed. Antidepressant drug effects on Olig1-expression were further explored in OLN-93 oligodendrocyte cultures. RESULTS: Nissl-stainings of both white matter regions showed a 19-27% reduction of total oligodendrocyte densities in MDD and BD, but not in SZ. In contrast, nuclear Olig1-immunoreactivity was elevated in MDD in the pACC-adjacent white matter (left: p = 0.008; right: p = 0.018); this effect tended to increase with antidepressant dosage (r = 0.631, p = 0.069). This reactive increase of Olig1 was confirmed by partly dose-dependent effects of imipramine and amitriptyline in oligodendrocyte cultures. Correspondingly, MBP expression in the pACC-adjacent white matter tended to increase with antidepressant dosage (r = 0.637, p = 0.065). Other tested brain regions showed no diagnosis-dependent differences regarding Olig1-immunoreactivity. CONCLUSIONS: Since nuclear Olig1-expression marks oligodendrocyte precursor cells, its increased expression along with reduced total oligodendrocyte densities (Nissl-stained) in the pACC-adjacent white matter of MDDpatients might indicate a (putatively medication-boosted) regenerative attempt to compensate oligodendrocyte loss.
Authors: Grazyna Rajkowska; Gouri Mahajan; Dorota Maciag; Monica Sathyanesan; Abiye H Iyo; Mohadetheh Moulana; Patrick B Kyle; William L Woolverton; Jose Javier Miguel-Hidalgo; Craig A Stockmeier; Samuel S Newton Journal: J Psychiatr Res Date: 2015-04-20 Impact factor: 4.791
Authors: K G Schraut; S B Jakob; M T Weidner; A G Schmitt; C J Scholz; T Strekalova; N El Hajj; L M T Eijssen; K Domschke; A Reif; T Haaf; G Ortega; H W M Steinbusch; K P Lesch; D L Van den Hove Journal: Transl Psychiatry Date: 2014-10-21 Impact factor: 6.222
Authors: Johann Steiner; Daniel Martins-de-Souza; Kolja Schiltz; Zoltan Sarnyai; Sabine Westphal; Berend Isermann; Henrik Dobrowolny; Christoph W Turck; Bernhard Bogerts; Hans-Gert Bernstein; Tamas L Horvath; Lorenz Schild; Gerburg Keilhoff Journal: Front Cell Neurosci Date: 2014-11-18 Impact factor: 5.505
Authors: Hans-Gert Bernstein; Gabriela Meyer-Lotz; Henrik Dobrowolny; Jana Bannier; Johann Steiner; Martin Walter; Bernhard Bogerts Journal: Front Cell Neurosci Date: 2015-08-10 Impact factor: 5.505
Authors: Peter Falkai; Johann Steiner; Berend Malchow; Jawid Shariati; Andreas Knaus; Hans-Gert Bernstein; Thomas Schneider-Axmann; Theo Kraus; Alkomiet Hasan; Bernhard Bogerts; Andrea Schmitt Journal: Front Cell Neurosci Date: 2016-03-30 Impact factor: 5.505