OBJECTIVE: To investigate the pathological effects on temporomandibular joint cartilage of an experimentally created unilateral anterior crossbite prosthesis. METHODS: Six-week-old female Sprague-Dawley rats were divided into experimental and control groups and further divided into three subgroups for three time points (n = 9). In experimental groups, the unilateral anterior crossbite was created by bonding two metal tubes on left maxillary and mandibular incisors. 2, 4, or 8 weeks after operation, the histomorphological changes and the expression changes of PCNA, COL II, aggrecan, MMP-3, MMP-9, MMP-13, and TIMP-1 in condylar cartilage were investigated by hematoxylin-eosin and toluidine blue staining, real-time PCR, and immunohistochemistry. RESULTS: In experimental groups, decreasing in chondrocytes, cell-free areas, reduction in the thickness of hypertrophic layer, and loss of extracellular matrix were noticed. The expression level of PCNA was decreased at week 2 and week 4. The mRNA expression of COL II was decreased at 4 week and that of aggrecan was increased at 2 week but decreased at 4 and 8 week. The expression of MMP-9, MMP-13, and TIMP-1 was increased at 2-week and decreased at 4- and 8-week, while that of MMP-3 was increased at 4- and 8-week. CONCLUSIONS: The present unilateral anterior crossbite prosthesis led to degradation of mandibular condylar cartilage.
OBJECTIVE: To investigate the pathological effects on temporomandibular joint cartilage of an experimentally created unilateral anterior crossbite prosthesis. METHODS: Six-week-old female Sprague-Dawley rats were divided into experimental and control groups and further divided into three subgroups for three time points (n = 9). In experimental groups, the unilateral anterior crossbite was created by bonding two metal tubes on left maxillary and mandibular incisors. 2, 4, or 8 weeks after operation, the histomorphological changes and the expression changes of PCNA, COL II, aggrecan, MMP-3, MMP-9, MMP-13, and TIMP-1 in condylar cartilage were investigated by hematoxylin-eosin and toluidine blue staining, real-time PCR, and immunohistochemistry. RESULTS: In experimental groups, decreasing in chondrocytes, cell-free areas, reduction in the thickness of hypertrophic layer, and loss of extracellular matrix were noticed. The expression level of PCNA was decreased at week 2 and week 4. The mRNA expression of COL II was decreased at 4 week and that of aggrecan was increased at 2 week but decreased at 4 and 8 week. The expression of MMP-9, MMP-13, and TIMP-1 was increased at 2-week and decreased at 4- and 8-week, while that of MMP-3 was increased at 4- and 8-week. CONCLUSIONS: The present unilateral anterior crossbite prosthesis led to degradation of mandibular condylar cartilage.
Authors: Christopher Phillip Chen; Jiehua Zhang; Bin Zhang; Mohamed G Hassan; Kyle Hane; Caroline C Chen; Ana Alejandra Navarro Palacios; Sunil Kapila; Andrew H Jheon; Alice F Goodwin Journal: JBMR Plus Date: 2022-07-03
Authors: M Zhang; H Wang; J Zhang; H Zhang; H Yang; X Wan; L Jing; L Lu; X Liu; S Yu; W Chang; M Wang Journal: Osteoarthritis Cartilage Date: 2015-12-31 Impact factor: 6.576
Authors: J Zhang; H Y Zhang; M Zhang; Z Y Qiu; Y P Wu; D A Callaway; J X Jiang; L Lu; L Jing; T Yang; M Q Wang Journal: Osteoarthritis Cartilage Date: 2014-04-03 Impact factor: 6.576